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  1. Link

    comt-antibody-epr6491b-ab124813.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-COMT antibody [EPR6491(B)] (ab124813)

  • Datasheet
  • SDS
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Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
  • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
  • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
  • Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)
  • OI-RD Scanning - Anti-COMT antibody [EPR6491(B)] (ab124813)
  • Anti-COMT antibody [EPR6491(B)] (ab124813)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6491(B)] to COMT
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
Product image
Anti-BDNF antibody [EPR1292] (ab108319)
Knockout
Product image
Human COMT knockout HEK-293T cell line (ab266537)

View more associated products

Overview

  • Product name

    Anti-COMT antibody [EPR6491(B)]
    See all COMT primary antibodies
  • Description

    Rabbit monoclonal [EPR6491(B)] to COMT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human COMT aa 250-350. The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293T, HAP1, A431 and MOLT4 cell lysates; Human fetal liver lysate. Flow Cyt: A431 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.01 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6491(B)
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Parkinson's disease
    • Other
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-COMT antibody [EPR6491(B)] - BSA and Azide free (ab248016)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human COMT knockout HEK-293T cell line (ab266537)
  • KO cell lysates

    • Human COMT knockout HEK-293T cell lysate (ab257396)
  • Positive Controls

    • A-431 membrane extract lysate (ab30134)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
  • Tissue specificity

    Brain, liver, placenta, lymphocytes and erythrocytes.
  • Sequence similarities

    Belongs to the mammalian catechol-O-methyltransferase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Cytoplasm and Cell membrane.
  • Target information above from: UniProt accession P21964 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1312 Human
    • Entrez Gene: 24267 Rat
    • Omim: 116790 Human
    • SwissProt: P21964 Human
    • SwissProt: P22734 Rat
    • Unigene: 370408 Human
    • Unigene: 704514 Human
    • Unigene: 220 Rat
    • Alternative names

      • Catechol O methyltransferase antibody
      • Catechol O-methyltransferase antibody
      • COMT antibody
      • COMT_HUMAN antibody
      • EC 2.1.1.6 antibody
      see all

    Images

    • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
      Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
      All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T cell lysate
      Lane 2 : COMT knockout HEK-293T cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 30 kDa
      Observed band size: 28 kDa why is the actual band size different from the predicted?



      Lanes 1-2: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab124813 Anti-COMT antibody [EPR6491(B)] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab124813 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
      Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
      All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : COMT knockout HAP1 whole cell lysate
      Lane 3 : A431 whole cell lysate
      Lane 4 : MOLT4 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 30 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE.  Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
      Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
      All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : A431 cell lysate
      Lane 3 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 30 kDa

    • Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)
      Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)
      ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
    • OI-RD Scanning - Anti-COMT antibody [EPR6491(B)] (ab124813)
      OI-RD Scanning - Anti-COMT antibody [EPR6491(B)] (ab124813)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-COMT antibody [EPR6491(B)] (ab124813)
      Anti-COMT antibody [EPR6491(B)] (ab124813)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab124813? Please let us know so that we can cite the reference in this datasheet.

    ab124813 has not yet been referenced specifically in any publications.

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