Recombinant Anti-COMT antibody [EPR6491(B)] (ab124813)
![Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)](https://www.abcam.com/ps/products/124/ab124813/Images/ab124813-388719-anti-comt-antibody-epr6491b-western-blot-hek293t-lysates.jpg "Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6491(B)] to COMT
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-COMT antibody [EPR6491(B)]
See all COMT primary antibodies -
Description
Rabbit monoclonal [EPR6491(B)] to COMT -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human COMT aa 250-350. The exact sequence is proprietary.
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Positive control
- WB: HEK-293T, HAP1, A431 and MOLT4 cell lysates; Human fetal liver lysate. Flow Cyt: A431 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.01 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6491(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
|
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| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa). |
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Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. -
Tissue specificity
Brain, liver, placenta, lymphocytes and erythrocytes. -
Sequence similarities
Belongs to the mammalian catechol-O-methyltransferase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1312 Human
- Entrez Gene: 24267 Rat
- Omim: 116790 Human
- SwissProt: P21964 Human
- SwissProt: P22734 Rat
- Unigene: 370408 Human
- Unigene: 704514 Human
- Unigene: 220 Rat
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Alternative names
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
see all
Images
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Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : COMT knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?Lanes 1-2: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control ab8245 observed at 37 kDa.
ab124813 Anti-COMT antibody [EPR6491(B)] was shown to specifically react with COMT in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266537 (knockout cell lysate ab257396) was used. Wild-type and COMT knockout samples were subjected to SDS-PAGE. ab124813 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)](https://www.abcam.com/ps/products/124/ab124813/Images/ab124813-270554-anti-comt-antibody-epr6491b-western-blot.jpg)
Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : COMT knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : MOLT4 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 30 kDaLanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)](https://www.abcam.com/ps/products/124/ab124813/Images/ab124813-138728-anti-comt-antibody-epr6491b-western-blot.jpg)
Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A431 cell lysate
Lane 3 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 30 kDa -
![Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)](https://www.abcam.com/ps/products/124/ab124813/Images/ab124813-138727-anti-comt-antibody-epr6491b-flow-cytometry.jpg)
Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry. -
![OI-RD Scanning - Anti-COMT antibody [EPR6491(B)] (ab124813)](https://www.abcam.com/ps/products/124/ab124813/Images/ab124813-138726-anti-comt-antibody-epr6491b.jpg)
OI-RD Scanning - Anti-COMT antibody [EPR6491(B)] (ab124813) -
![Anti-COMT antibody [EPR6491(B)] (ab124813)](https://www.abcam.com/ps/products/124/ab124813/Images/ab124813-6-benefits-of-recombinant-antibodies.png)
Anti-COMT antibody [EPR6491(B)] (ab124813)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124813 has not yet been referenced specifically in any publications.