Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-COMT antibody [EPR6491(B)] (ab124813)

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Overview

  • Product name

    Anti-COMT antibody [EPR6491(B)]
    See all COMT primary antibodies

  • Description

    Rabbit monoclonal [EPR6491(B)] to COMT

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide within Human COMT aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Human fetal Liver, A431 and MOLT4 cell lysates

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

    • Tissue specificity

      Brain, liver, placenta, lymphocytes and erythrocytes.

    • Sequence similarities

      Belongs to the mammalian catechol-O-methyltransferase family.

    • Post-translational
      modifications

      The N-terminus is blocked.

    • Cellular localization

      Cytoplasm and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Catechol O methyltransferase antibody
        • Catechol O-methyltransferase antibody
        • COMT antibody
        • COMT_HUMAN antibody
        • EC 2.1.1.6 antibody
        see all

      Images

      • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
        Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)

        Lane 1: Wild type HAP1 whole cell lysate (20 µg)
        Lane 2: COMT knockout HAP1 whole cell lysate (20 µg)
        Lane 3: A431 whole cell lysate (20 µg)
        Lane 4: MOLT4 whole cell lysate (20 µg)

        Lanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.

        ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE.  Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

      • Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
        Western blot - Anti-COMT antibody [EPR6491(B)] (ab124813)
        All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution

        Lane 1 : Human fetal Liver lysate
        Lane 2 : A431 cell lysate
        Lane 3 : MOLT4 cell lysate

        Lysates/proteins at 10 µg per lane.

        Developed using the ECL technique.

        Predicted band size: 30 kDa

      • Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)
        Flow Cytometry - Anti-COMT antibody [EPR6491(B)] (ab124813)
        ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
      • Other - Anti-COMT antibody [EPR6491(B)] (ab124813)
        Other - Anti-COMT antibody [EPR6491(B)] (ab124813)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      ab124813 has not yet been referenced specifically in any publications.

      Publishing research using ab124813? Please let us know so that we can cite the reference in this datasheet.

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