Product nameAnti-COMT antibody [EPR6491(B)]
See all COMT primary antibodies
DescriptionRabbit monoclonal [EPR6491(B)] to COMT
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human COMT aa 250-350. The exact sequence is proprietary.
- Human fetal Liver, A431 and MOLT4 cell lysates
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.01 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 24-30 kDa (predicted molecular weight: 30 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Tissue specificityBrain, liver, placenta, lymphocytes and erythrocytes.
Sequence similaritiesBelongs to the mammalian catechol-O-methyltransferase family.
modificationsThe N-terminus is blocked.
Cellular localizationCytoplasm and Cell membrane.
- Information by UniProt
- Catechol O methyltransferase antibody
- Catechol O-methyltransferase antibody
- COMT antibody
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: COMT knockout HAP1 whole cell lysate (20 µg)
Lane 3: A431 whole cell lysate (20 µg)
Lane 4: MOLT4 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab124813 observed at 28 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab124813 was shown to recognize COMT when COMT knockout samples were used, along with additional cross-reactive bands. Wild-type and COMT knockout samples were subjected to SDS-PAGE. Ab124813 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-COMT antibody [EPR6491(B)] (ab124813) at 1/1000 dilution
Lane 1 : Human fetal Liver lysate
Lane 2 : A431 cell lysate
Lane 3 : MOLT4 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 30 kDa
ab124813, at 1/10 dilution staining COMT in A431 cells, (Red) compared to a nonspecific negative control antibody (Green) by Flow Cytometry.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124813 has not yet been referenced specifically in any publications.