Key features and details
- Rabbit polyclonal to Conjugated Noradrenaline
- Suitable for: ELISA, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Conjugated Noradrenaline antibody
DescriptionRabbit polyclonal to Conjugated Noradrenaline
SpecificityUsing catecholamine-Glutaraldehyde-BSA conjugates, antibody specificity was performed with an ELISA test by competition experiments, as follows: (a) unconjugated or conjugated catecholamine concentration at half displacement (b) Non-reduced conjugate
Compound Cross-reactivity ratio (a) Noradrenaline 1 Octopamine 1/31 Dopamine 1/70 Adrenaline 1/177 Noradrenaline (b) 1/316 L-DOPA, Tyramine, unconjugated Noradrenaline 1/>5000
Tested applicationsSuitable for: ELISA, IHC-Frmore details
Species reactivityReacts with: Species independent
Synthetic noradrenaline conjugated to protein carriers (PC).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
PurityAmmonium Sulphate Precipitation
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6454 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: assay dependent.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
RelevanceNoradrenaline (NA) is a catecholamine neurotransmitter at postganglionic sympathetic fibres, produced following a series of steps from the essential amino acid phenylalanine. Terminal postganglionic sympathetic fibres have varicosities (where NA is synthesised and stored) which form synapses with the effector organ. The adrenal medulla is a major source of catecholamines (such as NA and adrenaline), however, most of the NA produced in the adrenal medulla is converted into adrenaline. NA is key in the fight-or-flight response and also directly increases heart rate, energy release from fat, and muscle readiness.
ab6454 has been referenced in 2 publications.
- Aschrafi A et al. Angiotensin II mediates the axonal trafficking of tyrosine hydroxylase and dopamine ß-hydroxylase mRNAs and enhances norepinephrine synthesis in primary sympathetic neurons. J Neurochem 150:666-677 (2019). PubMed: 31306490
- Arima Y et al. Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit. Elife 6:N/A (2017). PubMed: 28809157