Overview

  • Product name
    Anti-conjugated Octopamine antibody
  • Description
    Rabbit polyclonal to conjugated Octopamine
  • Host species
    Rabbit
  • Specificity
    This antibody targets conjugated octopamine. This antibody does not recognize free octopamine. Using a conjugate octopamine-protein carrier (Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: cross reactivity§ Octopamine-G-(Pc):1 Tyramine-G-(Pc):1/400 Naradrenaline-G-(Pc):1/10,000 Tyrosine-G-(Pc):1/>50,000 Dopamine-G-(Pc):1/100,000 G = Glutaraldehyde § Octopamine-G-(Pc) concentration/other conjugated catecholamine concentration at half displacement.
  • Tested applications
    Suitable for: IHC-Glut, ELISA, ICC, WBmore details
  • Species reactivity
    not applicable
  • Immunogen

    Chemical/ Small Molecule corresponding to conjugated Octopamine.

Properties

Applications

Our Abpromise guarantee covers the use of ab37092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Glut 1/1000 - 1/5000. Fixation should be done with gluatraldehyde. The use of PFA with glutaraldehyde may improve staining results.
ELISA 1/1000 - 1/5000.
ICC 1/1000 - 1/5000. Fixation should be done with gluatraldehyde. The use of PFA with glutaraldehyde may improve staining results.
WB 1/1000 - 1/2000. Predicted molecular weight: 1 kDa.

This antibody does not recognize free octopamine.

References

This product has been referenced in:
  • Châtel A  et al. Characterization of tyramine ß-hydroxylase, an enzyme upregulated by stress in Periplaneta americana. J Mol Endocrinol 50:91-102 (2013). ELISA . Read more (PubMed: 23197360) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

Unfortunately the supplying laboratory didn't have the image available for this product but we would still guarantee the product for use in WB.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us with your question. Our datasheet did say that formaldehyde is not a suitable fixative to use with this antibody, however I could not find any data regarding this statement. I have reviewed the notes from the testing laboratory, and they actually recommend using glutaraldehyde or PFA-glutaraldehyde to fix the tissue for optimal staining. I did not find evidence that Methacam or Zamboni were ever tested with this antibody, so I would suggest using one of the recommended fixatives if possible. Please note that this antibody only recognize the conjugated octopamine and will not recognize free octopamine. I have updated our datasheet with the information about the fixation method, and this change should be visible on our website shortly. I hope this information is helpful, but if you have any further questions please let me know and I'll be happy to help you.

Read More
Application
Immunohistochemistry (PFA fixed)
Sample
Fruit fly (Drosophila melanogaster) Cell (drosophila neuromuscular junction)
Specification
drosophila neuromuscular junction

Abcam user community

Verified customer

Submitted Feb 26 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Fruit fly (Drosophila melanogaster) Cultured Cells (cultured neurons)
Specification
cultured neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - PBST
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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