Key features and details
- Rabbit polyclonal to Connecdenn 3
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Connecdenn 3 antibody
DescriptionRabbit polyclonal to Connecdenn 3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Human Connecdenn 3 aa 601-801.
PRWQPDDKKLPEPEPQPLSLPSLQNASSLDATSSSKDSRSQLIPSESDQE VTSPSQSSTASADPSIWGDPKPSPLTEPLILHLTPSHKAAEDSTAQENPT PWLSTAPTEPSPPESPQILAPTKPNFDIAWTSQPLDPSSDPSSLEDPRAR PPKALLAERAHLQPREEPGALNSPATPTSNCQKSQPSSRPRVADLKKCFE
Database link: Q8IV53
- WB: Rat liver and lung lysates. IHC-P: Human lymph node and lung tissues.
Previously labelled as DENND1C.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab224721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 87 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionGuanine nucleotide exchange factor (GEF) for RAB35.
Sequence similaritiesContains 1 dDENN domain.
Contains 1 DENN domain.
Contains 1 uDENN domain.
Cellular localizationCytoplasm > cytosol. Cytoplasmic vesicle > clathrin-coated vesicle.
- Information by UniProt
- Connecdenn 3 antibody
- DEN1C_HUMAN antibody
- DENN domain containing 1C antibody
All lanes : Anti-Connecdenn 3 antibody (ab224721) at 1/500 dilution
Lane 1 : Rat liver lysate
Lane 2 : Rat lung lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87 kDa
Observed band size: 88 kDa why is the actual band size different from the predicted?
Paraffin-embedded human lymph node tissue stained for Connecdenn 3 using ab224721 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human lung tissue stained for Connecdenn 3 using ab224721 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224721 has not yet been referenced specifically in any publications.