Overview

  • Product name

    Anti-Connexin 32 / GJB1 antibody [EPR8036(2)]
    See all Connexin 32 / GJB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8036(2)] to Connexin 32 / GJB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Connexin 32/ GJB1 aa 50-150. The exact sequence is proprietary.
    Database link: P08034

  • Positive control

    • Human skeletal muscle, fetal liver, MCF-7 and Human stomach lysates; Human kidney tissue; MCF-7 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8036(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
    • Involvement in disease

      Defects in GJB1 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1) [MIM:302800]; also designated CMT-X. CMTX1 is a form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination, and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs and chronic axonal degeneration and regeneration on nerve biopsy. CMTX1 has both demyelinating and axonal features. Central nervous system involvement may occur.
      Defects in GJB1 may contribute to the phenotype of Dejerine-Sottas syndrome (DSS) [MIM:145900]; also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie-Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome.
    • Sequence similarities

      Belongs to the connexin family. Beta-type (group I) subfamily.
    • Cellular localization

      Cell membrane. Cell junction > gap junction.
    • Information by UniProt
    • Database links

    • Alternative names

      • Charcot Marie Tooth neuropathy X linked antibody
      • CMTX 1 antibody
      • CMTX antibody
      • CMTX1 antibody
      • Connexin-32 antibody
      • Connexin32 antibody
      • CX 32 antibody
      • Cx32 antibody
      • CXB1_HUMAN antibody
      • GAP junction 28 kDa liver protein antibody
      • Gap junction beta 1 protein antibody
      • Gap junction beta-1 protein antibody
      • Gap junction protein beta 1 32kD antibody
      • Gap junction protein beta 1 antibody
      • GJB 1 antibody
      • GJB1 antibody
      see all

    Images

    • All lanes : Anti-Connexin 32 / GJB1 antibody [EPR8036(2)] (ab181374) at 1/1000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Fetal liver lysate
      Lane 3 : MCF-7 lysate
      Lane 4 : Human stomach lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 32 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Connexin 32 / GJB1 with ab181374 at 1/100 dilution.

    • Flow cytometric analysis of MCF-7 cells labeling Connexin 32 / GJB1 with ab181374 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab181374 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab181374.
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