Immunohistochemistry (Frozen sections) abreview for Anti-Connexin 43 / GJA1 antibody [4E6.2]

Average
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Corneal epithelium)
Specification
Corneal epithelium
Fixative
Aceton-Methanol
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/250
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C

Secondary antibody

Name
Non-Abcam antibody was used: Alexa-Fluor 568 - Invitrogene
Host species: Mouse
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Dilution
1/500

Additional data

Additional Notes
We see quite well the junctions on the basal layer of the epithelium but also in the central cornea and in the limbus zone.

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Submitted Dec 10 2012

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