Immunohistochemistry (Frozen sections) abreview for Anti-Connexin 43 / GJA1 antibody [4E6.2]

Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample
Chicken Tissue sections (Hindbrain section)
Specification
Hindbrain section
Permeabilization
Yes - PBS + 0.1% Tween20
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (0.1%) + 3% BSA
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Rabbit anti-Mouse Alexa Fluor­ 568
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568
Dilution
1/1000

Additional data

Abcam response
Thank you for sharing this data with us. ab79010 has not previous been tested in IHC-Fr on Chicken and based on this abreview, it would appear that this antibody is not suitable for work on this species, under these conditions. Abcam would recommend ab117843 for IHC-Fr on Chicken. We hope this information is useful to our customers.

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Submitted Nov 28 2013

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