Key features and details
- Rabbit polyclonal to Connexin 43 / GJA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Connexin 43 / GJA1 antibody
See all Connexin 43 / GJA1 primary antibodies
DescriptionRabbit polyclonal to Connexin 43 / GJA1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Synthetic peptide. This information is considered to be commercially sensitive.
- ICC/IF: NIH/3T3 cells. Rat pup brain cells. IHC-P: Mouse heart tissue. WB: Mouse heart and rat brain tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 43 kDa.|
|IHC-P||1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 15 µg/ml.|
FunctionOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
Tissue specificityExpressed in the heart and fetal cochlea.
Involvement in diseaseDefects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
Sequence similaritiesBelongs to the connexin family. Alpha-type (group II) subfamily.
Cellular localizationCell membrane. Cell junction > gap junction.
- Information by UniProt
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized rat pup brain cells labeling Connexin 43 / GJA1 with ab235282 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green). The nuclear counterstain is DAPI (blue).
Immunohistochemical analysis of paraffin-embedded mouse heart tissue labeling Connexin 43 - GJA1 with ab235282 at 1/200 dilution (30 minutes at RT), followed by a ready to use anti-Rabbit polyclonal IgG (HRP) (8 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes.
Panel A: Mouse heart H&E.
Panel B: ab235282 at 20X magnification.
Panel C: ab235282 at 40X magnification.
All lanes : Anti-Connexin 43 / GJA1 antibody (ab235282) at 1/1000 dilution
Lane 1 : Mouse heart tissue
Lane 2 : Rat brain tissue
Lysates/proteins at 35 µg per lane.
All lanes : Goat anti-rabbit-HRP at 1/70000 dilution
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 0.5 secs.
Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling Connexin 43 / GJA1 with ab235282 at 15 µg/ml (Overnight at 4°C), followed by a Donkey anti-Rabbit IgG DyLight™488 conjugated preadsorbed secondary antibody at 5 µg/ml (1 hour at RT) (green). The nuclear counterstain is DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235282 has not yet been referenced specifically in any publications.