Anti-Connexin 43 / GJA1 antibody - C-terminal (ab219493)

Goat polyclonal Connexin 43 / GJA1 antibody. Validated in WB, IHC, ICC/IF and tested in Human, Monkey. Immunogen corresponding to recombinant fragment.

Overview

  • Product name

    Anti-Connexin 43 / GJA1 antibody - C-terminal
    See all Connexin 43 / GJA1 primary antibodies
  • Description

    Goat polyclonal to Connexin 43 / GJA1 - C-terminal
  • Host species

    Goat
  • Specificity

    This antibody detects endogenous levels of total Connexin 43 / GJA1
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human, Monkey
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Recombinant fragment within Rat Connexin 43/ GJA1 aa 230-382 (C terminal). The exact sequence is proprietary. Produced in E.coli.
    Database link: P08050

  • Positive control

    • WB - COS7 cell lysate ICC/IF - Primary RPE cells IHCP - Human uterus, endometrium tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab219493 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 43 kDa.
ICC/IF 1/50 - 1/250.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • Tissue specificity

    Expressed in the heart and fetal cochlea.
  • Involvement in disease

    Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • Sequence similarities

    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localization

    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

Images

  • Anti-Connexin 43 / GJA1 antibody - C-terminal (ab219493) at 1/1000 dilution + COS7 cell lysate at 100 µg

    Secondary
    Rabbit polyclonal to Goat IgG (HRP) at 1/10000 dilution

    Predicted band size: 43 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human uterus endometrium tissue labeling Connexin 43 / GJA1 with ab219493 at 10 µg/ml.

  • Immunofluorescent analysis of primary RPE cells labeling Connexin 43 / GJA1 with ab219493 at 1/100 dilution.

References

This product has been referenced in:

  • Li K  et al. Hypoxic Preconditioning Maintains GLT-1 Against Transient Global Cerebral Ischemia Through Upregulating Cx43 and Inhibiting c-Src. Front Mol Neurosci 11:344 (2018). Read more (PubMed: 30323740) »
See 1 Publication for this product

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