• Product name
    Anti-Connexin 43 / GJA1 antibody [CXN-6]
    See all Connexin 43 / GJA1 primary antibodies
  • Description
    Mouse monoclonal [CXN-6] to Connexin 43 / GJA1
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Cow, Cat, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Connexin 43/ GJA1 aa 362-381 conjugated to keyhole limpet haemocyanin.


  • General notes

    This product was changed from ascites to tissue culture supernatant on 24th August 2017. Lot GR231995 is from ascites, lot numbers higher than this will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

    For continuous use, store at 2–8 oC for up to one month. For extended storage, freeze in working aliquots. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.



Our Abpromise guarantee covers the use of ab11369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 23340590
WB 1/5000 - 1/7500. Detects a band of approximately 43 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/500 - 1/1000.


  • Function
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • Tissue specificity
    Expressed in the heart and fetal cochlea.
  • Involvement in disease
    Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • Sequence similarities
    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localization
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all


  • Immunocytochemistry/Immunofluorescence analysis of normoxic (A) and hypoxic (B) human mesenchymal stem cells labelling Connexin 43 / GJA1 with ab11369. Blue = DAPI (scale bars = 50 μm). Cells were grown on glass coverslips in 12-well tissue-culture dishes. Coverslips were washed with DPBS and fixed with 4% paraformaldehyde for 15 min, blocked, and incubated with the primary antibody for 1 hour at 37°C. Cells were washed and incubated with FITC-conjugated secondary antibody for 1 hour at 37°C.


This product has been referenced in:
  • Greiner J  et al. Confocal Microscopy-Based Estimation of Parameters for Computational Modeling of Electrical Conduction in the Normal and Infarcted Heart. Front Physiol 9:239 (2018). Read more (PubMed: 29670532) »
  • Wang Y  et al. The transplantation of Akt-overexpressing amniotic fluid-derived mesenchymal stem cells protects the heart against ischemia-reperfusion injury in rabbits. Mol Med Rep 14:234-42 (2016). WB ; Rabbit . Read more (PubMed: 27151366) »
See all 11 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Horse Tissue sections (aglandular stomach, epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
aglandular stomach, epithelium
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C

F Hernandez-Blazquez

Verified customer

Submitted Mar 16 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Horse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, pH 6
Blocking step
BSA as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Jul 08 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (heart, ventricular tissue)
Loading amount
60 µg
heart, ventricular tissue
Gel Running Conditions
Reduced Denaturing (4~12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 01 2008


Thank you for the details that you have provided and I'm sorry to hear that you're experiencing difficulty with ab11369. In order to help improve your results, I would like to suggest the following. For IHC, it is recommended to use this primary at a dilution of 1/8000, so I would suggest trying this dilution. For IHC-formalin fixed paraffin sections it is recommended to perform enzymatic antigen retrieval before commencing with IHC staining protocol (0.1% trypsin for 6-8 min at 37C). Use Standard ExtrAvidin- biotin immunoperoxidase technique procedure (Sigma Kit), and to block with 1% BSA. Have you tried this method? Please let me know if this helps and if you have any additional questions or concerns. If the suggestions do not help, and the antibody has been purchased in the past 90 days, I would be happy to offer a free of charge replacement or refund.

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