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Synthetic peptide corresponding to Connexin 43/ GJA1 aa 362-381 conjugated to keyhole limpet haemocyanin.
This product was changed from ascites to tissue culture supernatant on 24th August 2017. Lot GR231995 is from ascites, lot numbers higher than this will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
For continuous use, store at 2–8 oC for up to one month. For extended storage, freeze in working aliquots. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Our Abpromise guarantee covers the use of ab11369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23340590|
|WB||1/5000 - 1/7500. Detects a band of approximately 43 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/500 - 1/1000.|
Immunocytochemistry/Immunofluorescence analysis of normoxic (A) and hypoxic (B) human mesenchymal stem cells labelling Connexin 43 / GJA1 with ab11369. Blue = DAPI (scale bars = 50 μm). Cells were grown on glass coverslips in 12-well tissue-culture dishes. Coverslips were washed with DPBS and fixed with 4% paraformaldehyde for 15 min, blocked, and incubated with the primary antibody for 1 hour at 37°C. Cells were washed and incubated with FITC-conjugated secondary antibody for 1 hour at 37°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"