Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
Key features and details
- Rabbit polyclonal to Connexin 43 / GJA1 - Intercellular Junction Marker
- Suitable for: IHC-Fr, ICC, IHC-P, WB
- Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Monkey
- Isotype: IgG
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Overview
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Product name
Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker
See all Connexin 43 / GJA1 primary antibodies -
Description
Rabbit polyclonal to Connexin 43 / GJA1 - Intercellular Junction Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Monkey -
Immunogen
Synthetic peptide corresponding to Human Connexin 43/ GJA1 aa 362-382 (C terminal) conjugated to keyhole limpet haemocyanin.
Sequence:KPSSRASSRASSRPRPDDLEI
Database link: P17302 -
Positive control
- WB: Mouse brain and heart tissue extract; HEK-293, P19 and Rat2 cell lysates. IHC-P: Cow heart tissue; human heart and testis tissue; mouse heart muscle tissue. ICC: BHK cells. Human pluripotent stem cell derived cardiomyocyte.
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General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).
See other anti-rabbit secondary antibodies that can be used with this antibody.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity isolated antigen specific antibody is obtained by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to connexin 43. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
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| ICC | (1) |
1/400.
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| IHC-P | (19) |
1/1000 - 1/5000.
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| WB | (6) |
1/2000 - 1/8000. Detects a band of approximately 43 kDa.
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| Notes |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC
1/400. |
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IHC-P
1/1000 - 1/5000. |
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WB
1/2000 - 1/8000. Detects a band of approximately 43 kDa. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. -
Tissue specificity
Expressed in the heart and fetal cochlea. -
Involvement in disease
Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases. -
Sequence similarities
Belongs to the connexin family. Alpha-type (group II) subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 281193 Cow
- Entrez Gene: 403418 Dog
- Entrez Gene: 2697 Human
- Entrez Gene: 14609 Mouse
- Entrez Gene: 100518636 Pig
- Entrez Gene: 24392 Rat
- Omim: 121014 Human
- SwissProt: P18246 Cow
see all -
Alternative names
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
Immunohistochemical analysis of formalin-fixed, paraffion-embedded cow heart tissue staining Connexin 43 / GJA1 using ab11370 at1/2000 dilution. Detected using an Anti-Rabbit IgG-biotin antibody and an Avidin-peroxidase conjugate.
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Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)All lanes : Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/2000 dilution
Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : P19 (mouse embryonic carcinima cell line) cell lysate
Lane 3 : Rat2 (rat embryo-derived cell line) cell lysate
Lane 4 : MDCK (canine kidney cell line) cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG-peroxidase conjugate -
Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/8000 dilution + Mouse brain extract
Secondary
Anti-Rabbit IgG-AP conjugate with NBT/BCIP substrate -
Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)Immunocytochemical analysis of BHK (baby hamster kidney cell line) cells fixed and permeabilized with methanol followed by methanol:acetone. -Connexin 43 / GJA1 was labeled using ab11370 at 1/400 dilution, followed by a Goat Anti-Rabbit-FITC conjugate (Green). The nuclear counterstain is DAPI (Blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)Immunohistochemical analysis of formalin-fixed, paraffion-embedded human heart tissue staining Connexin 43 / GJA1 using ab11370 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG-FITC conjugate (Green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemical detection of Connexin 43 / GJA1 using ab11370 (1/1000) on human testis sections (PFA-fixed paraffin-embedded sections). Antigen retrieval : Heat mediated - Buffer/Enzyme Used: Citric acid pH6, 1% BSA as blocking agent for 10 mins @ rt°C. Biotin labelled secondary antibody used at 1/200. Stained are clusters of Leydig cells in the interstitium and what should be Sertoli cells within the seminiferous tubules and the junction between (?). Sertoli cells and tubule "capsular" cells are intensely positive at their membrane interfaces (capsular interface positivity only evident in upper middle of image). Interestingly,smooth muscle of human colon and myometrium were completely negative when tested at the same time.
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Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)Image courtesy of an anonymous Abreview.All lanes : Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/6000 dilution
Lane 1 : Whole tissue lysate prepared from murine left ventricle at 5 µg
Lane 2 : Whole tissue lysate prepared from murine left ventricle at 10 µg
Lane 3 : Whole tissue lysate prepared from murine left ventricle at 20 µg
Lane 4 : Whole tissue lysate prepared from murine left ventricle at 30 µg
Lane 5 : Whole tissue lysate prepared from murine left ventricle at 40 µg
Secondary
All lanes : HRP-conjugated donkey anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of an anonymous Abreviewab11370 staining Connexin 43 / GJA1 in human pluripotent stem cell derived cardiomyocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Clear antibody signal (yellow) at the interface of adjoining cells in a population of human embryonic stem cell derived cardiomyocytes (red = sarcomeric alpha actinin).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of Carl Hobbs, King's College London, United KingdomImmunohistochemistical detection of Connexin 43 / GJA1 using ab11370 on Formaldehyde-fixed paraffin-embedded mouse heart muscle sections. Antigen retrieval step: heat mediated using citric acid pH6. Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody ab11370 incubated at 1/2000 for 2 hours. Secondary antibody: anti rabbit IgG conjugated to biotin used at 1/200. Submitted image of cardiac myofibres in L/S shows clear, specific labelling of Intercalated discs which are rich in Gap junctions (Connexin 43 is a major component of Gap junctions). No other positivity is observed in mouse heart (the image is taken from a whole heart section). NB: in mouse tongue the ventral keratinising stratified squamous epithelium shows positivity in not only basal cells but also in the Prickle cell layer, although markedly reduced: data not shown but see here http://www.immunoportal.com/ for appropriate image.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (229)
ab11370 has been referenced in 229 publications.
- Potter JA et al. Collagen I Modifies Connexin-43 Hemichannel Activity via Integrin a2ß1 Binding in TGFß1-Evoked Renal Tubular Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33807408
- Takashima S et al. Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells. Sci Rep 11:1520 (2021). PubMed: 33452355
- Vink J et al. Extracellular Matrix Rigidity Modulates Human Cervical Smooth Muscle Contractility-New Insights into Premature Cervical Failure and Spontaneous Preterm Birth. Reprod Sci 28:237-251 (2021). PubMed: 32700284
- Çavdar S et al. Comparing astrocytic gap junction of genetic absence epileptic rats with control rats: an experimental study. Brain Struct Funct 226:2113-2123 (2021). PubMed: 34097147
- Zhao X et al. Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs. J Cell Mol Med 25:484-498 (2021). PubMed: 33205619