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  1. Link

    connexin-43--gja1-antibody-intercellular-junction-marker-ab11370.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Gap Junctions
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Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)

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  • SDS
Reviews (34)Q&A (5)References (229)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
  • Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
  • Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
  • Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
  • Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
  • Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)

Key features and details

  • Rabbit polyclonal to Connexin 43 / GJA1 - Intercellular Junction Marker
  • Suitable for: IHC-Fr, ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Monkey
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Connexin 43 / GJA1 antibody [EPR22955-101] (ab235585)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker
    See all Connexin 43 / GJA1 primary antibodies
  • Description

    Rabbit polyclonal to Connexin 43 / GJA1 - Intercellular Junction Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, ICC, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Cow, Dog, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human Connexin 43/ GJA1 aa 362-382 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    KPSSRASSRASSRPRPDDLEI


    Database link: P17302
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse brain and heart tissue extract; HEK-293, P19 and Rat2 cell lysates. IHC-P: Cow heart tissue; human heart and testis tissue; mouse heart muscle tissue. ICC: BHK cells. Human pluripotent stem cell derived cardiomyocyte.
  • General notes

    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

    Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077).

    See other anti-rabbit secondary antibodies that can be used with this antibody.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity isolated antigen specific antibody is obtained by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to connexin 43.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Gap Junctions
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell junction molecules
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (2)
Use at an assay dependent concentration.
ICC (1)
1/400.
IHC-P (19)
1/1000 - 1/5000.
WB (6)
1/2000 - 1/8000. Detects a band of approximately 43 kDa.
Notes
IHC-Fr
Use at an assay dependent concentration.
ICC
1/400.
IHC-P
1/1000 - 1/5000.
WB
1/2000 - 1/8000. Detects a band of approximately 43 kDa.

Target

  • Function

    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • Tissue specificity

    Expressed in the heart and fetal cochlea.
  • Involvement in disease

    Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • Sequence similarities

    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localization

    Cell membrane. Cell junction > gap junction.
  • Target information above from: UniProt accession P17302 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 281193 Cow
    • Entrez Gene: 403418 Dog
    • Entrez Gene: 2697 Human
    • Entrez Gene: 14609 Mouse
    • Entrez Gene: 100518636 Pig
    • Entrez Gene: 24392 Rat
    • Omim: 121014 Human
    • SwissProt: P18246 Cow
    • SwissProt: Q6S9C0 Dog
    • SwissProt: P17302 Human
    • SwissProt: P23242 Mouse
    • SwissProt: Q29101 Pig
    • SwissProt: P08050 Rat
    • Unigene: 74471 Human
    • Unigene: 378921 Mouse
    • Unigene: 10346 Rat
    see all
  • Alternative names

    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)

    Immunohistochemical analysis of formalin-fixed, paraffion-embedded cow heart tissue staining Connexin 43 / GJA1 using ab11370 at1/2000 dilution. Detected using an Anti-Rabbit IgG-biotin antibody and an Avidin-peroxidase conjugate.

  • Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    All lanes : Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/2000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : P19 (mouse embryonic carcinima cell line) cell lysate
    Lane 3 : Rat2 (rat embryo-derived cell line) cell lysate
    Lane 4 : MDCK (canine kidney cell line) cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG-peroxidase conjugate
  • Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/8000 dilution + Mouse brain extract

    Secondary
    Anti-Rabbit IgG-AP conjugate with NBT/BCIP substrate
  • Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)

    Immunocytochemical analysis of BHK (baby hamster kidney cell line) cells fixed and permeabilized with methanol followed by methanol:acetone. -Connexin 43 / GJA1 was labeled using ab11370 at 1/400 dilution, followed by a Goat Anti-Rabbit-FITC conjugate (Green). The nuclear counterstain is DAPI (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)

    Immunohistochemical analysis of formalin-fixed, paraffion-embedded human heart tissue staining Connexin 43 / GJA1 using ab11370 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG-FITC conjugate (Green).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

    Immunohistochemical detection of Connexin 43 / GJA1 using ab11370 (1/1000) on human testis sections (PFA-fixed paraffin-embedded sections). Antigen retrieval : Heat mediated - Buffer/Enzyme Used: Citric acid pH6, 1% BSA as blocking agent for 10 mins @ rt°C. Biotin labelled secondary antibody used at 1/200. Stained are clusters of Leydig cells in the interstitium and what should be Sertoli cells within the seminiferous tubules and the junction between (?). Sertoli cells and tubule "capsular" cells are intensely positive at their membrane interfaces (capsular interface positivity only evident in upper middle of image). Interestingly,smooth muscle of human colon and myometrium were completely negative when tested at the same time.

    See Abreview

  • Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Western blot - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)Image courtesy of an anonymous Abreview.
    All lanes : Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370) at 1/6000 dilution

    Lane 1 : Whole tissue lysate prepared from murine left ventricle at 5 µg
    Lane 2 : Whole tissue lysate prepared from murine left ventricle at 10 µg
    Lane 3 : Whole tissue lysate prepared from murine left ventricle at 20 µg
    Lane 4 : Whole tissue lysate prepared from murine left ventricle at 30 µg
    Lane 5 : Whole tissue lysate prepared from murine left ventricle at 40 µg

    Secondary
    All lanes : HRP-conjugated donkey anti-rabbit polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Observed band size: 43 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds

    See Abreview

  • Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Immunocytochemistry - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of an anonymous Abreview

    ab11370 staining Connexin 43 / GJA1 in human pluripotent stem cell derived cardiomyocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with saponin and blocked with 5% serum for 15 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    Clear antibody signal (yellow) at the interface of adjoining cells in a population of human embryonic stem cell derived cardiomyocytes (red = sarcomeric alpha actinin).

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker (ab11370)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
    Immunohistochemistical detection of  Connexin 43 / GJA1 using ab11370 on Formaldehyde-fixed paraffin-embedded mouse heart muscle sections. Antigen retrieval step: heat mediated using citric acid pH6. Blocking step: 1% BSA for 10 mins @ rt°C.  Primary antibody ab11370 incubated at 1/2000 for 2 hours.  Secondary antibody: anti rabbit IgG conjugated to biotin used at 1/200. Submitted image of cardiac myofibres in L/S shows clear, specific labelling of Intercalated discs which are rich in Gap junctions (Connexin 43 is a major component of Gap junctions). No other positivity is observed in mouse heart (the image is taken from a whole heart section). NB: in mouse tongue the ventral keratinising stratified squamous epithelium shows positivity in not only basal cells but also in the Prickle cell layer, although markedly reduced: data not shown but see here http://www.immunoportal.com/ for appropriate image.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (229)

Publishing research using ab11370? Please let us know so that we can cite the reference in this datasheet.

ab11370 has been referenced in 229 publications.

  • Potter JA  et al. Collagen I Modifies Connexin-43 Hemichannel Activity via Integrin a2ß1 Binding in TGFß1-Evoked Renal Tubular Epithelial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33807408
  • Takashima S  et al. Myocyte-specific enhancer factor 2c triggers transdifferentiation of adipose tissue-derived stromal cells into spontaneously beating cardiomyocyte-like cells. Sci Rep 11:1520 (2021). PubMed: 33452355
  • Vink J  et al. Extracellular Matrix Rigidity Modulates Human Cervical Smooth Muscle Contractility-New Insights into Premature Cervical Failure and Spontaneous Preterm Birth. Reprod Sci 28:237-251 (2021). PubMed: 32700284
  • Çavdar S  et al. Comparing astrocytic gap junction of genetic absence epileptic rats with control rats: an experimental study. Brain Struct Funct 226:2113-2123 (2021). PubMed: 34097147
  • Zhao X  et al. Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs. J Cell Mol Med 25:484-498 (2021). PubMed: 33205619
View all Publications for this product

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1-10 of 39 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (heart (cardiac muscle tissue))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
heart (cardiac muscle tissue)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 19 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spleen)
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 11 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (palatine tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
palatine tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 11 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 11 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 16 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH: 6.0
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Nov 14 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Immortalized Human Cardiomyocytes)
Permeabilization
Yes - Triton X-100
Specification
Immortalized Human Cardiomyocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Aug 10 2020

Western blot abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Immortalized Human Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
30 µg
Specification
Immortalized Human Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 10 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Connexin 43 / GJA1 antibody - Intercellular Junction Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human bone marrow derived stromal cells transplant)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
Human bone marrow derived stromal cells transplant
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 26 2020

Question

I have decided to take part in your testing discount program to test ab46758(Anti-c-kit antibody) in flow cytometry and IF.

I have some other questions:

Do Anti-GATA4 antibody [6H10] (ab86371),



Anti-Connexin 43 / GJA1 antibody (ab63851), Anti-Connexin 43 / GJA1 antibody (ab11370), Anti-CXCR4 antibody [44716.111] (ab10403), and Anti-CXCR4 antibody (ab1671),
reactwith Dog?

Ifyou do not have relevant empirical data,I would like very much to testthem in IF and submit feedback to you. Pleasegive meDISCOUNT CODE of these antibodys.

Sincerely Yours,

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Abcam community

Verified customer

Asked on Apr 12 2012

Answer

Thank you for your inquiry.

Unfortunately, the immunogen of ab1671 shows less than 60% homology with dog and therefore we do not expect a crpss-raction of this antibody. ab63851 does show a 93% homology with dog and would in general be eligable for the testing discount programme. But in this case ab11370 is the better choice since it shows 100% homology with dog and reacts also with Connexin 43 / GJA.

Please find the discount codes for ICC/IF and dog below:


ab46758:

DISCOUNT CODE: 100%xxxx

Expiration date: 12. August 2012

I am very pleased to hear you would like to accept our offer and test ab46758 in ICC/IF. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.



DISCOUNT CODEs:

ab10403 (whole protein immunogen 95% with dog): 100%xxxx


ab11370 (100% homology with dog): 100%xxxxx


ab86371 (93% homology with dog): 100%xxxx



Expiration date: 12. August 2012

I am very pleased to hear you would like to accept our offer and testthe above mentionend antibodiesin dog. This code will give you 1 freeprimary antibody per dicount code/tested antiboybefore the expiration date. To redeem this offer, please submit an Abreview for dog and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Abcam Scientific Support

Answered on Apr 12 2012

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