Overview

  • Product name

    Anti-Connexin 43 / GJA1 (phospho Y265) antibody
    See all Connexin 43 / GJA1 primary antibodies
  • Description

    Rabbit polyclonal to Connexin 43 / GJA1 (phospho Y265)
  • Host species

    Rabbit
  • Specificity

    ab193373 detects endogenous levels of Connexin 43 / GJA1 only when phosphorylated at tyrosine 265.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Connexin 43/ GJA1 aa 263-267 (phospho Y265) conjugated to keyhole limpet haemocyanin.
    Sequence:

    Q-K-Y(p)-A-Y


    Database link: P17302

  • Positive control

    • Extracts from HUVEC cells

Properties

Applications

Our Abpromise guarantee covers the use of ab193373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.

Target

  • Function

    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
  • Tissue specificity

    Expressed in the heart and fetal cochlea.
  • Involvement in disease

    Defects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
    Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
    Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
    Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
    Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
  • Sequence similarities

    Belongs to the connexin family. Alpha-type (group II) subfamily.
  • Cellular localization

    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links

  • Alternative names

    • Connexin 43 antibody
    • Connexin-43 antibody
    • Cx 43 antibody
    • Cx43 antibody
    • CXA1_HUMAN antibody
    • DFNB38 antibody
    • Gap junction 43 kDa heart protein antibody
    • Gap junction alpha-1 protein antibody
    • Gap junction protein alpha 1 43kDa (connexin 43) antibody
    • Gap junction protein alpha 1 43kDa antibody
    • Gap junction protein alpha like antibody
    • GJA 1 antibody
    • Gja1 antibody
    • GJAL antibody
    • ODD antibody
    • ODDD antibody
    • ODOD antibody
    • SDTY3 antibody
    see all

Images

  • All lanes : Anti-Connexin 43 / GJA1 (phospho Y265) antibody (ab193373) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with Antigen specific peptide

    Predicted band size: 43 kDa

References

ab193373 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat Connexin 43 Transiently Transfected into Madin)
Permeabilization
Yes - Acetone
Specification
Rat Connexin 43 Transiently Transfected into Madin
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone

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Verified customer

Submitted Oct 11 2017

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