Product nameAnti-Connexin 43 / GJA1 (phospho S368) antibody
See all Connexin 43 / GJA1 primary antibodies
DescriptionRabbit polyclonal to Connexin 43 / GJA1 (phospho S368)
SpecificitySpecific for ~43k Connexin43 protein phosphorylated at Ser368.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Non human primates
Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer368 of rat Connexin43.
- Rat hippocampal lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPrepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
Our Abpromise guarantee covers the use of ab30559 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 42 kDa).
The immunolabeling of Connexin43 is completely eliminated by treatment with lambda phosphatase.
FunctionOne gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph.
Tissue specificityExpressed in the heart and fetal cochlea.
Involvement in diseaseDefects in GJA1 are the cause of autosomal dominant oculodentodigital dysplasia (ODDD) [MIM:164200]; also known as oculodentoosseous dysplasia. ODDD is a highly penetrant syndrome presenting with craniofacial (ocular, nasal, dental) and limb dysmorphisms, spastic paraplegia, and neurodegeneration. Craniofacial anomalies tipically include a thin nose with hypoplastic alae nasi, small anteverted nares, prominent columnella, and microcephaly. Brittle nails and hair abnormalities of hypotrichosis and slow growth are present. Ocular defects include microphthalmia, microcornea, cataracts, glaucoma, and optic atrophy. Syndactyly type 3 and conductive deafness can occur in some cases. Cardiac abnormalities are observed in rare instances.
Defects in GJA1 are the cause of autosomal recessive oculodentodigital dysplasia (ODDD autosomal recessive) [MIM:257850].
Defects in GJA1 may be the cause of syndactyly type 3 (SDTY3) [MIM:186100]. Syndactyly is an autosomal dominant trait and is the most common congenital anomaly of the hand or foot. It is marked by persistence of the webbing between adjacent digits, so they are more or less completely attached. In this type there is usually complete and bilateral syndactyly between the fourth and fifth fingers. Usually it is soft tissue syndactyly but occasionally the distal phalanges are fused. The fifth finger is short with absent or rudimentary middle phalanx. The feet are not affected.
Defects in GJA1 are a cause of hypoplastic left heart syndrome (HLHS) [MIM:241550]. HLHS refers to the abnormal development of the left-sided cardiac structures, resulting in obstruction to blood flow from the left ventricular outflow tract. In addition, the syndrome includes underdevelopment of the left ventricle, aorta, and aortic arch, as well as mitral atresia or stenosis.
Defects in GJA1 are a cause of Hallermann-Streiff syndrome (HSS) [MIM:234100]. HSS is a disorder characterized by a typical skull shape (brachycephaly with frontal bossing), hypotrichosis, microphthalmia, cataracts, beaked nose, micrognathia, skin atrophy, dental anomalies and proportionate short stature. Mental retardation is present in a minority of cases.
Sequence similaritiesBelongs to the connexin family. Alpha-type (group II) subfamily.
Cellular localizationCell membrane. Cell junction > gap junction.
- Information by UniProt
- Connexin 43 antibody
- Connexin-43 antibody
- Cx 43 antibody
All lanes : Anti-Connexin 43 / GJA1 (phospho S368) antibody (ab30559) at 1/1000 dilution
Lane 1 : Rat hippocampal lysate
Lane 2 : Rat hippocampal lysate (lambda phosphatase treated)
Lysates/proteins at 10 µg per lane.
Predicted band size: 42 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
The phosphospecificity of this labeling is shown in the second lane which was incubated in lambda phosphatase (1200 units for 30 min) before being exposed to GJA1.
This product has been referenced in:
- Cotter ML et al. The lipidated connexin mimetic peptide SRPTEKT-Hdc is a potent inhibitor of Cx43 channels with specificity for the pS368 phospho-isoform. Am J Physiol Cell Physiol 317:C825-C842 (2019). Read more (PubMed: 31365296) »
- Yin X et al. Roles of astrocytic connexin-43, hemichannels, and gap junctions in oxygen-glucose deprivation/reperfusion injury induced neuroinflammation and the possible regulatory mechanisms of salvianolic acid B and carbenoxolone. J Neuroinflammation 15:97 (2018). Read more (PubMed: 29587860) »