Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2] (ab78408)
Key features and details
- Mouse monoclonal [5B9.2] to Connexin 45/GJA7/Cx45
- Suitable for: Flow Cyt, ICC
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2]
See all Connexin 45/GJA7/Cx45 primary antibodies -
Description
Mouse monoclonal [5B9.2] to Connexin 45/GJA7/Cx45 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse, Cow, Dog, Pig, Chinese hamster -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Epitope
Near C terminal, cytoplasmic. -
Positive control
- ICC/IF: Connexin 45/GJA7/Cx45 transfected rat C6 cells. Flow Cyt: HeLa cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 17th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.0536% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
5B9.2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC |
Use at an assay dependent concentration.
|
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC
Use at an assay dependent concentration. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. -
Sequence similarities
Belongs to the connexin family. Gamma-type subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 100689357 Chinese hamster
- Entrez Gene: 511119 Cow
- Entrez Gene: 490936 Dog
- Entrez Gene: 10052 Human
- Entrez Gene: 14615 Mouse
- Entrez Gene: 100048959 Pig
- Entrez Gene: 266706 Rat
- Omim: 608655 Human
see all -
Alternative names
- Connexin 45 / GJA7 antibody
- Connexin 45 antibody
- Connexin-45 antibody
see all
Images
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ab78408 staining Connexin 45/GJA7/Cx45 transfected rat C6 cells by Immunocytochemistry.
This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab78408 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78408, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab78408 has been referenced in 7 publications.
- Zhou E et al. Vascular smooth muscle cell phenotypic transition regulates gap junctions of cardiomyocyte. Heart Vessels 35:1025-1035 (2020). PubMed: 32270355
- Lee J et al. In vitro generation of functional murine heart organoids via FGF4 and extracellular matrix. Nat Commun 11:4283 (2020). PubMed: 32883967
- Xiao H et al. Transcription factor Tbx18 induces the differentiation of c-kit+ canine mesenchymal stem cells (cMSCs) into SAN-like pacemaker cells in a co-culture model in vitro. Am J Transl Res 10:2511-2528 (2018). PubMed: 30210689
- Wan HJ et al. Propofol-induced vasodilation of mesenteric arterioles via BKCa channel and gap junction. Exp Ther Med 16:2960-2968 (2018). PubMed: 30233668
- Liu H et al. Role of gap junctions in the contractile response to agonists in the mesenteric resistance artery of rats with acute hypoxia. Mol Med Rep 15:1823-1831 (2017). PubMed: 28259990
- Dovmark TH et al. Connexin-43 channels are a pathway for discharging lactate from glycolytic pancreatic ductal adenocarcinoma cells. Oncogene 36:4538-4550 (2017). WB . PubMed: 28368405
- Li D et al. Connexins and steroidogenesis in mouse Leydig cells. Can J Physiol Pharmacol 91:157-64 (2013). PubMed: 23458200