Overview

  • Product name

    Anti-Connexin 45/GJA7/Cx45 antibody [5B9.2]
    See all Connexin 45/GJA7/Cx45 primary antibodies
  • Description

    Mouse monoclonal [5B9.2] to Connexin 45/GJA7/Cx45
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, ICCmore details
  • Species reactivity

    Reacts with: Rat, Rabbit, Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human Connexin 45/GJA7/Cx45 aa 354-367 conjugated to keyhole limpet haemocyanin.
    Sequence:

    QAYSHQNNPHGPRE

  • Epitope

    Near C terminal, cytoplasmic.
  • Positive control

    • ICC/IF: Connexin 45/GJA7/Cx45 transfected rat C6 cells. FC: HeLa cells.
  • General notes

    Previously labelled as Connexin 45 / GJA7. 

    This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab78408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

WB Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC Use at an assay dependent concentration.

Target

Images

  • ab78408 staining Connexin 45/GJA7/Cx45 transfected rat C6 cells by Immunocytochemistry.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing HeLa cells stained with ab78408 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78408, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Wan HJ  et al. Propofol-induced vasodilation of mesenteric arterioles via BKCa channel and gap junction. Exp Ther Med 16:2960-2968 (2018). Read more (PubMed: 30233668) »
  • Dovmark TH  et al. Connexin-43 channels are a pathway for discharging lactate from glycolytic pancreatic ductal adenocarcinoma cells. Oncogene 36:4538-4550 (2017). WB . Read more (PubMed: 28368405) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - other (muscle)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
30 µg
Specification
muscle
Blocking step
Blocking buffer for fluorescent western blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Miss. Nathalie Da Silva

Verified customer

Submitted Jul 13 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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