Anti-Connexin 45/GJA7/Cx45 antibody (ab135474)
Key features and details
- Rabbit polyclonal to Connexin 45/GJA7/Cx45
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Connexin 45/GJA7/Cx45 antibody
See all Connexin 45/GJA7/Cx45 primary antibodies -
Description
Rabbit polyclonal to Connexin 45/GJA7/Cx45 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Connexin 45/GJA7/Cx45 (C terminal).
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Positive control
- Rat heart extracts and Human brain tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 49% PBS
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab135474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 45 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. -
Sequence similarities
Belongs to the connexin family. Gamma-type subfamily. -
Cellular localization
Cell membrane. Cell junction > gap junction. - Information by UniProt
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Database links
- Entrez Gene: 10052 Human
- Entrez Gene: 14615 Mouse
- Entrez Gene: 266706 Rat
- Omim: 608655 Human
- SwissProt: P36383 Human
- SwissProt: P28229 Mouse
- SwissProt: A4GG66 Rat
- Unigene: 712052 Human
see all -
Alternative names
- Connexin 45 / GJA7 antibody
- Connexin 45 antibody
- Connexin-45 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab135474 has been referenced in 6 publications.
- Lozic M et al. Alteration of Cx37, Cx40, Cx43, Cx45, Panx1, and Renin Expression Patterns in Postnatal Kidneys of Dab1-/- (yotari) Mice. Int J Mol Sci 22:N/A (2021). PubMed: 33525532
- Kosovic I et al. Spatio-temporal patterning of different connexins in developing and postnatal human kidneys and in nephrotic syndrome of the Finnish type (CNF). Sci Rep 10:8756 (2020). PubMed: 32471989
- Kosovic I et al. Connexin Signaling in the Juxtaglomerular Apparatus (JGA) of Developing, Postnatal Healthy and Nephrotic Human Kidneys. Int J Mol Sci 21:N/A (2020). PubMed: 33172216
- Juric M et al. Expression of Connexins 37, 43 and 45 in Developing Human Spinal Cord and Ganglia. Int J Mol Sci 21:N/A (2020). PubMed: 33302507
- Zhang Y et al. Transcriptome Landscape of Human Folliculogenesis Reveals Oocyte and Granulosa Cell Interactions. Mol Cell 72:1021-1034.e4 (2018). PubMed: 30472193
- Wu A et al. Effect of Wenxin Granules on Gap Junction and MiR-1 in Rats with Myocardial Infarction. Biomed Res Int 2017:3495021 (2017). PubMed: 29094045