Overview

  • Product name

    Anti-Connexin 45/GJA7/Cx45 antibody
    See all Connexin 45/GJA7/Cx45 primary antibodies
  • Description

    Rabbit polyclonal to Connexin 45/GJA7/Cx45
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Connexin 45/GJA7/Cx45 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab91444)

  • Positive control

    • This antibody gave a positive signal in Rat Lung Tissue Lysate and in the following whole cell lysates: SHSY5Y; SKNBE; SKNSH.
  • General notes

    Previously labelled as Connexin 45 / GJA7. 

Properties

Applications

Our Abpromise guarantee covers the use of ab70365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 45 kDa).

Target

Images

  • All lanes : Anti-Connexin 45/GJA7/Cx45 antibody (ab70365) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 41 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 35 kDa, 68 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes
  • Anti-Connexin 45/GJA7/Cx45 antibody (ab70365) at 1 µg/ml + Lung (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 20 minutes

References

This product has been referenced in:

See 1 Publication for this product

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