Anti-Constitutive androstane receptor antibody (ab186869)

Overview

  • Product name
    Anti-Constitutive androstane receptor antibody
    See all Constitutive androstane receptor primary antibodies
  • Description
    Rabbit polyclonal to Constitutive androstane receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human Constitutive androstane receptor aa 103-352 (C terminal).
    Database link: Q14994

  • Positive control
    • U87 and BT474 cell lysates; Mouse liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab186869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 40 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function
    Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
  • Tissue specificity
    Predominantly expressed in liver.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed by a short N-terminal domain followed by the DNA binding, hinge, and ligand binding/dimerization domains.
  • Post-translational
    modifications
    Phosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Recruited to the cytoplasm by DNAJC7.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAR antibody
    • CAR BETA antibody
    • CAR1 antibody
    • CARA antibody
    • Constitutive activator of retinoid response antibody
    • constitutive active receptor antibody
    • Constitutive active response antibody
    • constitutive androstane nuclear receptor variant 2 antibody
    • constitutive androstane nuclear receptor variant 3 antibody
    • constitutive androstane nuclear receptor variant 4 antibody
    • constitutive androstane nuclear receptor variant 5 antibody
    • Constitutive androstane receptor antibody
    • MB67 antibody
    • MGC97144 antibody
    • NR1I3 antibody
    • NR1I3_HUMAN antibody
    • Nuclear receptor subfamily 1 group I member 3 antibody
    • orphan nuclear hormone receptor antibody
    • Orphan nuclear receptor MB67 antibody
    • Orphan nuclear receptor NR1I3 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling Constitutive androstane receptor with ab186869 at 1/100. Magnification: 400x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophagus tissue labelling Constitutive androstane receptor with ab186869 at 1/100. Magnification: 400x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human esophageal cancer tissue labelling Constitutive androstane receptor with ab186869 at 1/100. Magnification: 400x.
  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186869. Blue DAPI for nuclear staining.
  • All lanes : Anti-Constitutive androstane receptor antibody (ab186869) at 1/500 dilution

    Lane 1 : U87 cell lysate
    Lane 2 : BT474 cell lysate
    Lane 3 : Mouse liver tissue lysate

    Predicted band size: 40 kDa

References

This product has been referenced in:
  • Wang X  et al. Constitutive androstane receptor activation promotes bilirubin clearance in a murine model of alcoholic liver disease. Mol Med Rep 15:3459-3466 (2017). Read more (PubMed: 28393244) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Pig Cell lysate - whole cell (Brain endothelial cells)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Brain endothelial cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Yu Siong Ho

Verified customer

Submitted May 08 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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