Overview

  • Product name
    Anti-Constitutive androstane receptor antibody
    See all Constitutive androstane receptor primary antibodies
  • Description
    Rabbit polyclonal to Constitutive androstane receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Chimpanzee
  • Immunogen

    Synthetic peptide: MASREDELRN CVVCGDQATG YHFNALTCEG CKGFFRRTVS KSIGPTCPFA, corresponding to a region within internal sequence amino acids 1-50 of Human Constitutive androstane receptor

  • Positive control
    • Human liver tissue. Fetal liver cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Function
    Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
  • Tissue specificity
    Predominantly expressed in liver.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed by a short N-terminal domain followed by the DNA binding, hinge, and ligand binding/dimerization domains.
  • Post-translational
    modifications
    Phosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Recruited to the cytoplasm by DNAJC7.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAR antibody
    • CAR BETA antibody
    • CAR1 antibody
    • CARA antibody
    • Constitutive activator of retinoid response antibody
    • constitutive active receptor antibody
    • Constitutive active response antibody
    • constitutive androstane nuclear receptor variant 2 antibody
    • constitutive androstane nuclear receptor variant 3 antibody
    • constitutive androstane nuclear receptor variant 4 antibody
    • constitutive androstane nuclear receptor variant 5 antibody
    • Constitutive androstane receptor antibody
    • MB67 antibody
    • MGC97144 antibody
    • NR1I3 antibody
    • NR1I3_HUMAN antibody
    • Nuclear receptor subfamily 1 group I member 3 antibody
    • orphan nuclear hormone receptor antibody
    • Orphan nuclear receptor MB67 antibody
    • Orphan nuclear receptor NR1I3 antibody
    see all

Images

  • All lanes : Anti-Constitutive androstane receptor antibody (ab62590) at 1.25 µg/ml

    All lanes : Rat whole liver tissue lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-rabbit IgG polyclonal

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible non-specific binding)


    Exposure time: 5 minutes

    See Abreview

  • Anti-Constitutive androstane receptor antibody (ab62590) at 1.25 µg/ml + fetal liver cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 40 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Antibody diluted in 5% skim milk / PBS buffer. Gel concentration: 12%.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling Constitutive androstane receptor with ab62590 at 4-8µg/ml. Arrows indicate positively labelled hepatocytes. Magnification: 400X.

References

This product has been referenced in:
  • Yang T  et al. Quercetin-3-O-ß-D-glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats. Mol Med Rep 18:2599-2612 (2018). Read more (PubMed: 30015887) »
  • Hudson GM  et al. Constitutive androstane receptor regulates the intestinal mucosal response to injury. Br J Pharmacol 174:1857-1871 (2017). Read more (PubMed: 28320072) »
See all 2 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
Western blot
Sample
Mouse Cell lysate - whole cell (liver tissue lysate)
Gel Running Conditions
Reduced Denaturing (8% polyacrylamide gel)
Loading amount
30 µg
Specification
liver tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Sara De Martin

Verified customer

Submitted Jun 26 2015

Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10% polyacrylamide)
Sample
Rat Tissue lysate - whole (Liver tissue lysate)
Specification
Liver tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Sara De Martin

Verified customer

Submitted Oct 07 2013

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% Acrylamide resolving gel)
Sample
Rat Tissue lysate - whole (Human HepG2 cells, Rat B-13 cells and liver lysate)
Specification
Human HepG2 cells, Rat B-13 cells and liver lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 04 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (Skin Fibroblasts)
Loading amount
20 µg
Specification
Skin Fibroblasts
Gel Running Conditions
Reduced Denaturing (12.5)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 07 2013

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