Key features and details
- Goat polyclonal to Contactin 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Contactin 1 antibody
See all Contactin 1 primary antibodies
DescriptionGoat polyclonal to Contactin 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Cow, Pig, Chimpanzee, Monkey, Gorilla
- Human brain cerebellum tissue; Human brain cerebellum, Mouse and Rat brain tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 113 kDa.|
FunctionContactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
Tissue specificityStrongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
Involvement in diseaseDefects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia.
Sequence similaritiesBelongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- CNTN 1 antibody
- CNTN antibody
- Cntn1 antibody
Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain cerebellum tissue labeling Contactin 1 with ab191285 at 5 µg/ml.
All lanes : Anti-Contactin 1 antibody (ab191285) at 0.3 µg/ml
Lane 1 : Human cerebellum tissue lysate in RIPA buffer at 35 µg
Lane 2 : Mouse brain tissue lysate in RIPA buffer at 35 µg
Lane 3 : Rat brain tissue lysate in RIPA buffer
Predicted band size: 113 kDa
The observed molecular weight corresponds to the glycosylated form.
ab191285 has not yet been referenced specifically in any publications.