Anti-Contactin 1 antibody (ab191285)
Key features and details
- Goat polyclonal to Contactin 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Contactin 1 antibody
See all Contactin 1 primary antibodies -
Description
Goat polyclonal to Contactin 1 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Guinea pig, Cow, Pig, Chimpanzee, Monkey, Gorilla -
Immunogen
Synthetic peptide corresponding to Human Contactin 1 aa 300-400 (internal sequence) (Cysteine residue). (NP_001834.2; NP_778203.1; NP_001242992.1).
Database link: Q12860 -
Positive control
- Human brain cerebellum tissue; Human brain cerebellum, Mouse and Rat brain tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 113 kDa.
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Notes |
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IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 113 kDa. |
Target
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Function
Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth. -
Tissue specificity
Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle. -
Involvement in disease
Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia. -
Sequence similarities
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 451836 Chimpanzee
- Entrez Gene: 281705 Cow
- Entrez Gene: 100731131 Guinea pig
- Entrez Gene: 1272 Human
- Entrez Gene: 101047133 Monkey
- Entrez Gene: 103238160 Monkey
- Entrez Gene: 12805 Mouse
- Entrez Gene: 100521555 Pig
see all -
Alternative names
- CNTN 1 antibody
- CNTN antibody
- Cntn1 antibody
see all
Images
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Immunohistochemical analysis of formalin fixed, paraffin embedded Human brain cerebellum tissue labeling Contactin 1 with ab191285 at 5 µg/ml.
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All lanes : Anti-Contactin 1 antibody (ab191285) at 0.3 µg/ml
Lane 1 : Human cerebellum tissue lysate in RIPA buffer at 35 µg
Lane 2 : Mouse brain tissue lysate in RIPA buffer at 35 µg
Lane 3 : Rat brain tissue lysate in RIPA buffer
Predicted band size: 113 kDaThe observed molecular weight corresponds to the glycosylated form.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab191285 has been referenced in 1 publication.
- Appeltshauser L et al. Diabetes Mellitus Is a Possible Risk Factor for Nodo-paranodopathy With Antiparanodal Autoantibodies. Neurol Neuroimmunol Neuroinflamm 9:N/A (2022). PubMed: 35314491