Overview

  • Product name

  • Description

    Goat polyclonal to Contactin 1
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human Contactin 1 aa 858-870 (Cysteine residue).
    Sequence:

    C-QVTSQEYSARLEN


    Database link: Q12860

  • Positive control

    • IHC-P: Mouse cerebral cortex. WB: Mouse and rat brain lysate. Human cerebellum lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.3 - 1 µg/ml.

Target

  • Function

    Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
  • Tissue specificity

    Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
  • Involvement in disease

    Defects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. Contactin family.
    Contains 4 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CNTN 1 antibody
    • CNTN antibody
    • Cntn1 antibody
    • CNTN1_HUMAN antibody
    • Contactin-1 antibody
    • Contactin1 antibody
    • F3 antibody
    • F3cam antibody
    • Glycoprotein gp135 antibody
    • gp 135 antibody
    • GP135 antibody
    • MYPCN antibody
    • Neural cell surface protein F3 antibody
    see all

Images

  • Paraffin embedded human cerebellum tissue stained for Contactin 1 with ab223217 at 5 µg/ml. DAB-staining.

  • Anti-Contactin 1 antibody (ab223217) at 1 µg/ml + human cerebellum lysate in RIPA buffer at 35 µg


    Primary incubation: 1 hour

  • All lanes : Anti-Contactin 1 antibody (ab223217) at 1 µg/ml

    Lane 1 : Mouse brain lysate in RIPA buffer
    Lane 2 : Rat brain lysate in RIPA buffer

    Lysates/proteins at 35 µg per lane.


    Primary incubation: 1 hour

  • Paraffin embedded mouse cerebral cortex tissue stained for Contactin 1 with ab223217 at 5 µg/ml. DAB-staining.

References

ab223217 has not yet been referenced specifically in any publications.

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