Key features and details
- Goat polyclonal to Contactin 1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Contactin 1 antibody
See all Contactin 1 primary antibodies
DescriptionGoat polyclonal to Contactin 1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- IHC-P: Mouse cerebral cortex. WB: Mouse and rat brain lysate. Human cerebellum lysate.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab223217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.3 - 1 µg/ml.
1 hour primary incubation is recommended for this product.
FunctionContactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
Tissue specificityStrongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
Involvement in diseaseDefects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia.
Sequence similaritiesBelongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- CNTN 1 antibody
- CNTN antibody
- Cntn1 antibody
Paraffin embedded human cerebellum tissue stained for Contactin 1 with ab223217 at 5 µg/ml. DAB-staining.
Anti-Contactin 1 antibody (ab223217) at 1 µg/ml + human cerebellum lysate in RIPA buffer at 35 µg
Primary incubation: 1 hour
All lanes : Anti-Contactin 1 antibody (ab223217) at 1 µg/ml
Lane 1 : Mouse brain lysate in RIPA buffer
Lane 2 : Rat brain lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Primary incubation: 1 hour
Paraffin embedded mouse cerebral cortex tissue stained for Contactin 1 with ab223217 at 5 µg/ml. DAB-staining.
ab223217 has not yet been referenced specifically in any publications.