Key features and details
- Rabbit polyclonal to Contactin 1 - C-terminal
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Contactin 1 antibody - C-terminal
See all Contactin 1 primary antibodies
DescriptionRabbit polyclonal to Contactin 1 - C-terminal
Tested applicationsSuitable for: IHC-Frmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human, Rhesus monkey
Recombinant fragment within Human Contactin 1 (C terminal). The exact sequence is proprietary.
Database link: Q12860
- IHC-Fr: Embryonic mouse brain and adult mouse retina tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/100 - 1/1000.|
FunctionContactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
Tissue specificityStrongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
Involvement in diseaseDefects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia.
Sequence similaritiesBelongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- CNTN 1 antibody
- CNTN antibody
- Cntn1 antibody
Frozen sections of embryonic mouse brain (mE18.5) tissue stained for Contactin 1 (red) using ab227491 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: DAPI (blue).
Frozen sections of adult mouse retina tissue stained for Contactin 1 (green) using ab227491 at 1/250 dilution in immunohistochemical analysis.
Counterstain: beta Tubulin 3, stained by beta Tubulin 3 antibody (red) at 1/250 dilution.
Nuclear counterstain: DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227491 has not yet been referenced specifically in any publications.