Anti-Contactin 6 antibody (ab224596)
Key features and details
- Rabbit polyclonal to Contactin 6
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Contactin 6 antibody -
Description
Rabbit polyclonal to Contactin 6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment corresponding to Human Contactin 6 aa 700-850.
Database link: Q9UQ52 -
Positive control
- WB: A549 and HepG2 cell lysate. IHC: Human thyroid tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/500 - 1/5000. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Function
Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination. -
Tissue specificity
Expressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord. -
Sequence similarities
Belongs to the immunoglobulin superfamily. Contactin family.
Contains 4 fibronectin type-III domains.
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 27255 Human
- Entrez Gene: 27256 Rat
- Omim: 607220 Human
- SwissProt: Q9UQ52 Human
- SwissProt: P97528 Rat
- Unigene: 387300 Human
- Unigene: 10644 Rat
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Alternative names
- CNTN 6 antibody
- CNTN6 antibody
- CNTN6_HUMAN antibody
see all
Images
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All lanes : Anti-Contactin 6 antibody (ab224596) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa -
Paraffin-embedded human thyroid tissue stained for Contactin 6 using ab224596 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab224596 has not yet been referenced specifically in any publications.