Abcam antibody training on how to choose and use antibodies

​​​Get all the essentials for using antibodies in various immunoassays with the Abcam antibody basics training series, with a certificate at the end.

Whether you are asking questions about antibody structure and the difference between monoclonal vs polyclonal, seeking guidance in choosing primary and secondary antibodies, or trying to validate your antibody, join our free on-demand training to gain full confidence in choosing and using antibodies.

Enroll for free in Abcam antibody basics training.


Participants’ reviews

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‘…the course resources are excellent.’​​

‘It's worth recommending.’

Training structure

Our online training will guide you from choosing the right antibody for your experiment, handling and storing antibodies through to validation, choosing the appropriate controls, optimizing your protocol, and troubleshooting. Whether you are unsure about the difference between antigen vs antibody or wonder how to choose a high-quality antibody with minimal batch-to-batch variability, we got you covered.

The Abcam antibody basics training is divided into 4 parts:

  • Part 1 teaches you how to choose the right primary and secondary antibodies for your experiment. You’ll also learn about antibody structure, 5 different classes of antibodies (called antibody isotypes), and the difference between monoclonal vs polyclonal vs recombinant antibodies.
  • Part 2 focuses on how to properly store and handle your antibodies, including those in conjugated formats. Also, you’ll find an answer to the most important question of all: “Help! I left my antibody out on the bench overnight – will it still work?”
  • Part 3 explains how to validate antibodies in your experimental setup, choose the appropriate controls, and optimize your protocol. You’ll also gain access to step-by-step protocols for IHC, ICC/IF, western blot, and flow cytometry, which will guide you through sample preparation, incubation, and visualization of your data.
  • Part 4 helps you troubleshooting the most common problems you might encounter in immunoassays such as IHC, ICC/IF, western blot, and flow cytometry. This will include the following problems: no/weak signal, high background, and incorrect staining.

Upon completion of your training, you can test your knowledge with our final quiz and get a certificate.

Enroll now and instantly access all training materials.

Get the most out of your immunoassays by becoming a confident antibody user.

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​Training value

Abcam antibody basics training covers all the essential information you’ll need to properly choose and use antibodies in various immunoassays.

At the end of this online training, you’ll be able to

  • Understand antibody structure, including the difference between F(ab) and Fc regions as well as between light and heavy chains
  • Comprehend the advantages and disadvantages of polyclonal and monoclonal antibodies
  • Select the right primary and secondary antibody for your experiment
  • Properly store and handle your antibodies
  • Validate your antibody in your setup
  • Choose the appropriate controls
  • Optimize and apply protocols for IHC, ICC, western blot, and flow cytometry
  • Troubleshoot the most common problems in IHC, ICC, western blot and flow cytometry

Sign up now for Abcam antibody basics training.

Already know your antibodies? Check out our training programs on fluorescent imaging, western blot, flow cytometry, IHC, or ChIP.