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A GFP antibody can be helpful for amplifying low GFP signal or running protein pulldown assays. Our rabbit polyclonal anti-GFP antibody (ab290) has been tested in flow cytometry, IHC, ICC, IP, and WB and is suitable for ChIP.
Customer data using our anti-GFP (ab290) antibody. A: posterior view showing CD8::GFP localized to the calyx and nls.dsRED in Kenyon cell nuclei, ab290 used at 1:20,000. B: GFP-transfected NIH3T3 cells, ab290 used at 1:200. Bi: basal GFP expression levels in the same sample (as B) - no antibody applied. Bii: non-tramnsfected NIH3T3 cells (control). C: mouse brain sections, anti-NeuN (red), DAPI (blue), and anti-GFP (ab290, used at 1:1,000) staining of GFP-cre (green, yellow with NeuN colocalization).
Anti-GFP (ab290) has been used successfully by hundreds of independent researchers, is the most highly-cited anti-GFP antibody on CiteAb (over 1,000 citations), and continues to be widely-cited year-on-year.
Our anti-GFP antibody (ab290) has also received in excess of 100 five-star customer reviews and is highly rated across all validated applications.
ChIP = chromatin immunoprecipitation, ICC/IF = immunocytochemistry/immunofluorescence, IHC = immunohistochemistry, IP = immunoprecipitation, Flow = flow cytometry, WB = western blot.
A monoclonal alternative
An alternative to our highly successful ab290, rabbit polyclonal anti-GFP antibody, is our range of RabMAb® rabbit monoclonal antibodies. These offer the superior antigen recognition of the rabbit immune system combined with the specificity and consistency of a monoclonal.
Customer data using out RabMAb® anti-GFP antibodies. Top left: BHK cells transfected with GFP-Sec61 beta, ab183734 used at 1:1,000. Bottom left: mouse pancreas, ab183734 used at 1:100. Ttop right: mouse lung tumor tissue, ab183734 used at 1:250. Bottom right: MDBK cells transfected with LAMP1-EGFP, ab183734 used at 1:2,000.