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With just 30 seconds of hands-on time, we introduce a faster and more efficient way of barcoding antibodies with oligonucleotides.
Recently developed techniques, such as CITE-seq, REAP-seq, and Cell hashing, are becoming increasingly popular for exploring single-cell multi-omic approaches. By combining barcoded antibodies with scRNA-seq platforms (i.e.10x Genomics Chromium, BD Rhapsody™ Single-Cell Analysis System, Fluidigm C1, Drop-seq, Clontech iCell8, and ddSEQ™ ) we are now able to correlate transcriptomics data with the actual protein expression in a single cell.
However, one of the biggest challenges with these techniques is how to efficiently conjugate oligonucleotides to antibodies.
With Lightning-Link® antibody barcoding kits you can conjugate your preferred antibody to the pre-activated oligo mix with just 30 seconds hands-on time. This will enable you to explore more parameters at a single-cell level while not being limited by commercially available antibody-oligo conjugates.
Lightning-Link® antibody barcoding kit workflow
You might also be interested in our Oligonucleotide Conjugation Kit. It enables simple and rapid conjugation of antibodies to oligonucleotides at the desired ratio, with a high recovery of materials and a superior clean up procedure.