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Fas (Apo-1/CD95/TNFRSF6) is a type 1 transmembrane receptor that belongs to the tumor necrosis factor (TNF) receptor superfamily. Members of this family act as sensors of the extracellular environment and signal through a variety of intracellular factors to give rise to appropriate cellular responses1. Structurally, the protein is approximately 37kDa in mass, consists of 335 amino acids, and is expressed in various cell types and tissues, including thymocytes, activated T cells, hepatocytes, heart, and kidney2.
Fas is a death receptor that mediates programmed cell death to maintain immune homeostasis. Interaction between Fas and its ligand, FasL, activates caspase-8 which in turn signals through downstream effector caspases to initiate apoptosis3.
Paraformaldehyde fixed frozen mouse spleen tissue section (tissue fixed for 1 hour in 4% PFA, 20% sucrose at RT) stained for Fas (green) using ab82419 at 1/50 dilution in immunohistochemical analysis. An Invitrogen Goat anti Rabbit Alexa Fluor® 488 was used as a secondary antibody at 1/1000 dilution. Counterstained with Hoechst 33258 (blue) for 10mins at RT. Primary antibody blocked using 10% goat serum for 30 min at RT.
Immunohistochemistry (Frozen sections) - Anti-Fas antibody (ab82419)
Image courtesy of an anonymous Abreview
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD95 with ab133619 antibody at a dilution of 1/250.
Heat -mediated antigen -retrieval was performed prior to IHC staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR5700] (ab133619)