Fas

Fas (Apo-1/CD95/TNFRSF6) is a type 1 transmembrane receptor that belongs to the tumor necrosis factor (TNF) receptor superfamily. Members of this family act as sensors of the extracellular environment and signal through a variety of intracellular factors to give rise to appropriate cellular responses¹. Structurally, the protein is approximately 37kDa in mass, consists of 335 amino acids, and is expressed in various cell types and tissues, including thymocytes, activated T cells, hepatocytes, heart, and kidney².

Fas is a death receptor that mediates programmed cell death to maintain immune homeostasis.  Interaction between Fas and its ligand, FasL, activates caspase-8, which in turn signals through downstream effector caspases to initiate apoptosis³.

Applications

​Our Fas antibodies, kits, and proteins are validated for use in a variety of key applications including ​western blot, immunocytochemistry, immunoprecipitation, and immunohistochemistry.

​	Paraformaldehyde- fixed frozen mouse spleen tissue section (tissue fixed for 1 hour in 4% PFA, 20% sucrose at RT) stained for Fas (green) using ab82419 at 1/50 dilution in immunohistochemical analysis. An Invitrogen Goat anti- Rabbit Alexa Fluor® 488 was used as a secondary antibody at 1/1000 dilution. Counterstained with Hoechst 33258 (blue) for 10mins at RT. Primary antibody blocked using 10% goat serum for 30 min at RT.
Immunohistochemistry (Frozen sections) - Anti-Fas antibody (ab82419) Image courtesy of an anonymous Abreview

​	Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD95 with ab133619 antibody at a dilution of 1/250. Heat -mediated antigen -retrieval was performed prior to IHC staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR5700] (ab133619)