Fluorescent imaging training
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Become a cell imaging expert with Abcam training series on fluorescent imaging, with a certificate at the end. Whether you are embarking on your first set of fluorescent imaging experiments, looking for the detailed cell or tissue imaging protocols, or troubleshooting your last experiment, join our free on-demand training to gain full confidence in fluorescent imaging.
Enroll for free training on fluorescent cell imaging
Participants' reviews
‘The content is very detailed and informative, [a] great help to my follow-up experiments.’
‘Clear & sharp knowledge about the topic.’
Training structure
From imaging basics and ICC/IF and IHC protocols, to troubleshooting and answers to the most FAQs around immunofluorescence, our online training will teach you how to run your experiments and help troubleshoot them.
The Abcam fluorescent imaging course is divided into 4 parts:
- Part 1 introduces you to microscopy and cell counting basics such as using a confocal microscope and a hemocytometer as well as implementing a good aseptic technique for cell imaging.
- Part 2 teaches you how to choose the right antibody for your fluorescent imaging experiment. You’ll also learn the difference between direct and indirect staining methods and get a guide for choosing a suitable fluorophore or multiple fluorophores for a multiplex.
- Part 3 covers the ICC and IHC protocols, from sample preparation to immunostaining and mounting.
- Part 4 helps you to improve your fluorescence microscopy images by troubleshooting the most common problems you might encounter in the lab when running ICC and IHC.
Upon completion of your training, you can test your knowledge with our final quiz and get a certificate.
Enroll now and instantly access all training materials.
Get the best fluorescence microscopy images.
Figure 1. On the left panel, ICC/IF staining on SV40LT-SMC cells, with anti-GAPDN antibody (ab8245) in green and anti-alpha tubulin antibody (ab202272), microtubule marker, in red. On the right panel, ICC/IF staining on SV40LT-SMC cells, with anti-alpha tubulin antibody [DM1-A] (ab 7291) in green.
Training value
Our online fluorescent imaging training covers all the key information you’ll need to get started with your fluorescent experiments and become an imaging expert.
At the end of this online training, you’ll be fully versed in
- Microscopy and cell counting basics
- Choosing the right antibody for your fluorescent cell imaging experiments
- ICC and IHC protocols
- Multi-fluorescent analysis
- Troubleshooting IHC and ICC experiments
Improve your fluorescence microscopy images with our free course on Fluorescent cell imaging.
Happy imaging and check out our other online courses.