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Green fluorescent protein (GFP) is a β-barrel-shaped protein1 consisting of 238 amino acids and with a molecular weight of ~27kDa. GFP is isolated from the crystal jellyfish Aequorea victoria, and its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light via energy transfer2. GFP is excited at a wavelength of 488nm and has an emission peak at ~507nm. However, high-resolution crystal structures of GFP allow scientists to manipulate its protein structure and engineer color variants that emit at different wavelengths3,4.
Our range of anti-GFP antibodies, proteins, and ELISA kits are validated for a variety of key applications including western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation (ChIP), flow cytometry, ELISA, and immunoprecipitation.
|Immunohistochemistry - Free Floating - Anti-GFP antibody - ChIP Grade (ab290)|
ab13970 staining mouse olfactory bulb tissue sections by IHC-Fr.
Sections were PFA fixed, permeabilized in 0.4% Triton-X and blocked with 5% serum for 2 hours at 25°C. The primary antibody was diluted 1/1000 and incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-chicken was used as the secondary antibody.
|Immunohistochemistry (Frozen sections) - Anti-GFP antibody (ab13970)|
|Anti-GFP antibody [EPR14104] (ab183734)||ICC/IF, Flow Cyt|
|Anti-GFP antibody (ab13970)||ICC/IF, IHC-Fr|
|Anti-GFP antibody - ChIP Grade (ab290)||WB, ChIP|
|Anti-GFP antibody (FITC) (ab6662)||ICC/IF|
|Anti-GFP antibody (Biotin) (ab6658)||IHC-P|
|Anti-GFP antibody (HRP) (ab6663)||WB|
|ELISA and Assay kits||Sensitivity||Detection|
|GFP ELISA kit, fluorescent (ab229403)**|
0.9 pg/ml - 2000 pg/ml
|GFP ELISA kit (ab171581) *||2.7 pg/ml - 2000 pg/ml (Colorimetric)||Colorimetric|
|GFP quantification kit (ab235672)|
0.01 µg/ml - 10 µg/ml
|Recombinant A. victoria GFP protein (ab84191)|
|Recombinant GFP protein (His tag) (ab134853)|
*This kit is part of the SimpleStep ELISA® range.
**This kit is part of the CatchPoint® SimpleStep ELISA® range.