Immunohistochemistry (IHC) training
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Achieve the best possible results in tissue immunostaining with the Abcam immunohistochemistry training series, with a certificate at the end. Whether you need to lay foundations in immunohistochemistry or take your experiments up a notch, join our free on-demand training to make IHC staining easy.
Participants’ reviews
‘…the course resources are excellent.’
‘The content is very detailed and informative, [a] great help to my follow-up experiments.’
Immunohistochemistry training structure
Our training starts with essentials like sample preparation and antibody selection and then guides you through immunostaining protocols and troubleshooting.
Our immunohistochemistry training is divided into 4 parts:
- Part 1 lays the foundation for a successful IHC staining. You’ll learn about sample preparation, fixation, embedding, and sectioning.
- Part 2 takes you through key immunostaining protocol steps such as antigen retrieval, permeabilization, and blocking. You’ll also learn how to select primary and secondary antibodies as well as the appropriate detection system.
- Part 3 teaches you how to perform counterstaining, multicolor staining, and finally how to mount your slides to get those beautiful IHC images you’ve always dreamed of. You’ll also find out how to choose the appropriate controls.
- Part 4 provides troubleshooting tips for the most common problems and issues you’re likely to encounter during your IHC experiments.
Upon training completion, you can test your knowledge with our final quiz and get a certificate.
Enroll now and instantly access all training materials.
Achieve accurate and reliable results with IHC staining.
Figure 1. On the left, fluorescence multiplex IHC staining of normal human tonsil tissue (formalin-fixed paraffin-embedded section). Merged staining of anti-PD1 (ab237728; orange; Opal™520), anti-PDL1 (ab237726; green; Opal™540), anti-CD68 (ab192847; yellow; Opal™570), anti-CD3 (ab16669; red; Opal™620), anti-Ki67 (ab16667; light blue; Opal™650) and anti-PanCK (ab7753; grey; Opal™690). On the right, IHC staining with anti-Ki67 antibody (ab16667) in a section of formalin-fixed paraffin-embedded normal human tonsil.
Immunohistochemistry training value
Our free on-demand training provides you with all essentials for tissue immunostaining.
At the end of this training, you’ll be fully equipped to run your next IHC experiment and get those crisp and accurate IHC images. You’ll learn:
- The two main methods of preserving tissues for IHC
- Tissue fixation and embedding methods
- The two approaches to antigen retrieval
- The differences between direct and indirection detection, as well as chromogenic and fluorescent labels
- The key considerations for choosing primary and secondary antibodies
- How to design multicolor experiments
- How to choose the appropriate controls
- Troubleshooting tips for some of the most common problems with IHC staining
Sign up now for our free on-demand training on tissue immunostaining.
Already know IHC staining? Check out our Antibody basics training, Fluorescent imaging training, or Western blot training.