Essential antibody guides

Essential guides to choosing and using antibodies for new researchers.

We know that as a new researcher you have a lot of questions, especially when it comes to figuring out which antibody technique to use: Shall I use a polyclonal? Do I need to validate this? Which type of antibody should I use for western blotting? Fortunately, we have the answers to those questions, and we've gathered them all in one place for you. Which is nice. Now you can get right to planning your experiments.


Poly or mono?
Learn the difference between monoclonal and polyclonal antibodies and when using one over the other might be a good idea.


Which secondary?
The secondary you pick can influence your results. Our guide will help you choose the most appropriate secondary antibody for your application.


Antibodies vs applications
It's important to be sure that your antibody is suited to the intended application, and that the application itself can address your questions. We can help you match antibodies to applications.


Validation
To have complete confidence in your results, you need to validate your antibodies. This guide highlights the pros and cons or various validation techniques


Western blot protocol
A powerful technique to quantify proteins, use this protocol for a step-by-step to running your own western blots.


IHC guide
From sample prep to staining, mounting and trouble shooting, this guide covers everything you might need to get to grips with immunohistochemistry.


Imaging reagents
With so many imaging reagents, it can be difficult to select the best one. Here we detail the most popular resource for cell imaging and include includes protocol tips, webinars, and product guides.