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Having to trawl through old protocols from manuals, your lab's former post-docs, or cryptic messages from your supervisor can be a painful endeavor. To combat this, we've pulled out the most useful protocols we can think of so that you can just jump straight into running your experiments and getting results.
This includes solutions and reagents, procedures, and useful links to guide you through your experiment.
Step-by-step IHC protocols for paraffin, frozen, and free-floating sections, plus a helpful guide on signal amplification.
A complete protocol for IP that includes reagents and a table to help you choose the correct protein beads.
In situ hybridization
From probe choice to DIG-labeling, this protocol takes you through ISH step-by-step.