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Vascular Endothelial Growth Factor A (VEGFA) is a highly conserved, 27kDa dimeric glycoprotein, which belongs to the VEGF family alongside VEGFB, VEGFC, VEGFD, and placenta growth factor (PIGF)1. Common isoforms, generated as the result of alternative splicing include VEGFA121, VEGFA165, VEGFA189, and VEGFA206, which contain 121, 165, 189, and 206 amino acids respectively2.
VEGFA is secreted by many cell types such as endothelial cells as well as tumors, and was originally shown to be an endothelial growth factor as well as a regulator of vascular permeability3,4. As VEGFA is a crucial regulator of angiogenesis, which accompanies growth of solid tumors, the targeting of VEGFA has revealed innovative therapeutic approaches in oncology, with the first VEGFA inhibitor approved in 2004 for the first-line treatment of metastatic colorectal cancer5.
Our range of anti-VEGFA antibodies, proteins, and ELISA kits are validated for use in a variety of key applications including western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation (ChIP), flow cytometry, immunoprecipitation, and ELISA.
|||Purified ab52917 staining VEGF in NIH/3T3 (Mouse embryo fibroblast cell line) cells.The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 min and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with purified ab52917 at 5 μg/ml and ab195884, at 1/250 dilution, overnight at +4°C, followed by a further incubation at room temperature for 1h with a Goat anti-Rabbit Alexa Fluor®488 secondary (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labeled in blue with DAPI.|
|Immunocytochemistry/ Immunofluorescence - Anti-VEGFA antibody [EP1176Y] (ab52917)|
IHC image of ab1316 staining VEGF in human cerebellum formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab1316, 5 μg/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
|Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGFA antibody [VG-1] (ab1316)|
|Anti- VEGFA antibodies||Applications||Species reactivity|
|Anti-VEGFA antibody [VG-1] (ab1316)||ELISA, ICC, ICC/IF, IHC-FoFr, IHC-P, IP, WB||Dog, human, mouse, rat, rabbit, rat|
|Anti-VEGFA antibody (ab46154)||ELISA, WB||Human, mouse, rat|
|Anti-VEGFA antibody (ab51745)||ELISA, IHC-P, WB||Human, mouse|
|Anti-VEGFA antibody [EP1176Y] - C-terminal (ab52917)||Flow Cyt, ICC/IF, IHC-P, IP, WB||Human, mouse|
|Anti-VEGFA antibody [EP1176Y] - BSA and Azide free (ab185238)||Flow Cyt, ICC/IF, IHC-P, IP, WB||Human, mouse|
|Anti-VEGFA antibody [EP1176Y] (Phycoerythrin) (ab209439)||Flow Cyt||Human|
|Anti-VEGFA antibody [EP1176Y] (Alexa Fluor® 594) (ab206888)||ICC/IF||Human|
|ELISA and assay kits||Sensitivity range||Detection|
|Human VEGF ELISA Kit (ab222510)*||12.5 pg/ml - 800 pg/ml||Colorimetric|
|Mouse VEGF ELISA Kit (ab209882)*||5.8 pg/ml - 370 pg/ml||Colorimetric|
|Human VEGFA ELISA Kit (ab119566)||15.6 pg/ml - 1000 pg/ml||Colorimetric|
|Human VEGF ELISA Kit (ab100662)||8.23 pg/ml - 6000 pg/ml||Colorimetric|
|Rat VEGF ELISA Kit (ab100786)||0.82 pg/ml - 200 pg/ml||Colorimetric|
|Horse VEGFA ELISA kit (ab272034)||4.1 pg/ml - 1000 pg/ml||Colorimetric|
|Recombinant Rabbit VEGFA protein (ab189228)||SDS-PAGE|
|Recombinant Human VEGFA protein (ab55566)||SDS-PAGE, WB|