Western blot training
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Become a pro in western blot analysis with the Abcam training series on western blot, with a certificate at the end. Whether you are embarking on your first western blot experiment, looking for detailed western bot protocols, or troubleshooting your last experiment, our free on-demand training will give you full confidence in western blot analysis.
Participants’ reviews
‘…the course resources are excellent.’
‘…a comprehensive introduction to the experiment technology.’
Training structure
From basic principles and essential steps of the western blot protocol to optimization and more advanced techniques, our online training will teach you how to run your western blot experiments and help troubleshoot them. Whether you are questioning what western blotting is or trying to optimize your next experiment, our training has got you covered.
Our western blot training is divided into 4 parts:
- Part 1 guides you through western blot basics, from choosing the right antibody and the appropriate controls to sample preparation. You’ll also get recipes for the essential western blot buffers and solutions.
- Part 2 takes you through all steps of western blot protocol, including gel electrophoresis, protein transfer and visualization, and membrane stripping for restaining.
- Part 3 helps you troubleshoot the most common problems you might encounter in western blot. You’ll also gain access to step-by-step western blot protocols for phosphorylated proteins and histones.
- Part 4 explains the advantages of fluorescent western blotting and how to make the switch from chemiluminescence to fluorescence. We’ll also guide you through a detailed fluorescent western blot protocol and share some troubleshooting tips.
Upon training completion, you can test your knowledge with our final quiz and get a certificate.
Enroll now and instantly access all training materials.
Discover your best work with straightforward blotting.
Figure 1. Western blot with Anti-PKC alpha antibody (ab32376). Green - ab32376 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa. ab32376 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined.
Training value
At the end of this online training, you’ll be fully equipped to run the entire western blot experiment. You’ll also have a good understanding of:
- Basic principles of western blot analysis
- Selecting the right antibodies for your western blot experiment
- Including the appropriate controls for western blot analysis
- The advantages of fluorescence vs ECL
- Implementing fluorescent western blot protocol
- Western blotting with phosphorylated proteins and histones
- Troubleshooting the most common problems in western blot
Boost your essential skills with our western blot training.
Have you already mastered western blot analysis? Check out our training programs on antibody basics, fluorescent imaging, IHC, flow cytometry, and ChIP.