For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Become a pro in western blot analysis with the Abcam training series on western blot, with a certificate at the end. Whether you are embarking on your first western blot experiment, looking for detailed western bot protocols, or troubleshooting your last experiment, our free on-demand training will give you full confidence in western blot analysis.
‘…the course resources are excellent.’
‘…a comprehensive introduction to the experiment technology.’
From basic principles and essential steps of the western blot protocol to optimization and more advanced techniques, our online training will teach you how to run your western blot experiments and help troubleshoot them. Whether you are questioning what western blotting is or trying to optimize your next experiment, our training has got you covered.
Our western blot training is divided into 4 parts:
Upon training completion, you can test your knowledge with our final quiz and get a certificate.
Discover your best work with straightforward blotting.
Figure 1. Western blot with Anti-PKC alpha antibody (ab32376). Green - ab32376 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa. ab32376 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined.
At the end of this online training, you’ll be fully equipped to run the entire western blot experiment. You’ll also have a good understanding of: