Coomassie brilliant blue R-250, SDS-PAGE and Bradford Assay dye (ab146260)

Overview

  • Product name

    Coomassie brilliant blue R-250, SDS-PAGE and Bradford Assay dye
  • Description

    SDS-PAGE and Bradford Assay dye.
  • Alternative names

    • Acid Blue 83
    • Brilliant blue R
    • Brilliant Indocyanine 6B
    • CI 42660
  • Biological description

    Triphenylmethane dye used in SDS-PAGE for the analysis of proteins. The dye molecule binds to proteins to form a protein-dye complex. This complex stabilizes the negatively charged anionic form of the dye producing the blue color, even under acidic conditions. Also used in the Bradford Assay to quantify the concentration of protein in a solution, such as cell lysates.

  • CAS Number

    6104-59-2
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    N-[4-[[4-[(4-Ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfobenzenemethanaminium inner salt monosodium salt
  • Molecular weight

    825.99
  • Molecular formula

    C45H44N3NaO7S2
  • Storage instructions

    Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 10 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Choi JK & Yoo GS Fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counter ion-dyes, Coomassie brilliant blue R-250 and neutral red. Arch Pharm Res 25:704-8 (2002). Read more (PubMed: 12433209) »
  • Chen H  et al. One-step Coomassie brilliant blue R-250 staining of proteins in polyacrylamide gel. Anal Biochem 212:295-6 (1993). Read more (PubMed: 7690194) »
  • Dzandu JK  et al. Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique. Proc Natl Acad Sci U S A 81:1733-7 (1984). Read more (PubMed: 6200882) »

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