Key features and details
- Rabbit polyclonal to COP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-COP1 antibody
See all COP1 primary antibodies
DescriptionRabbit polyclonal to COP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
A region between residue 1 and 50 of human COP1. (NP_071902.2)
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).|
|IP||Use a concentration of 2 - 5 µg/ml.|
FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
Tissue specificityUbiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the COP1 family.
Contains 1 RING-type zinc finger.
Contains 7 WD repeats.
DomainThe RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
Cellular localizationNucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
- Information by UniProt
- Constitutive photomorphogenesis protein 1 homolog antibody
- COP antibody
- E3 ubiquitin-protein ligase RFWD2 antibody
All lanes : Anti-COP1 antibody (ab70889) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 3 minutes
HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70889 at 1 µg/ml for WB, subsequent to IP at 3 µg/mg lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab70889 has been referenced in 1 publication.
- Ren X et al. TRB3 stimulates SIRT1 degradation and induces insulin resistance by lipotoxicity via COP1. Exp Cell Res 382:111428 (2019). PubMed: 31125554