The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 5 µg/ml.
Is unsuitable for WB.
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
Protein modification; protein ubiquitination.
Belongs to the COP1 family. Contains 1 RING-type zinc finger. Contains 7 WD repeats.
The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
HeLa whole cell lysate (1 mg for IP, 20% of IP loaded). Antibodies:
Lane 1: ab70889 at 3µg/ml for IP.
Lane 2: ab70890 at 3µg/ml for IP.
Lane 3: ab70891 at 3µg/ml for IP.
Lane 4: IgG control
Subsequent Western Blotting was carried out using ab70889 at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.