Key features and details
- Rabbit polyclonal to COPB2
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-COPB2 antibody
See all COPB2 primary antibodies
DescriptionRabbit polyclonal to COPB2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey, Orangutan
Recombinant fragment corresponding to Human COPB2 aa 657-906.
AYQLAVEAESEQKWKQLAELAISKCQFGLAQECLHHAQDYGGLLLLATAS GNANMVNKLAEGAERDGKNNVAFMSYFLQGKVDACLELLIRTGRLPEAAF LARTYLPSQVSRVVKLWRENLSKVNQKAAESLADPTEYENLFPGLKEAFV VEEWVKETHADLWPAKQYPLVTPNEERNVMEEGKDFQPSRSTAQQELDGK PASPTPVIVASHTANKEEKSLLELEVDLDNLELEDIDTTDINLDEDILDD
Database link: P35606
- WB: Human placenta tisue lysate; MCF7, HeLa, HepG2 and Jurkat whole cell lysates. IHC-P: Human spleen and placenta tissue.
This product was previously labelled as beta COP I
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 102 kDa (predicted molecular weight: 102, 99 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionThe coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.
Sequence similaritiesBelongs to the WD repeat COPB2 family.
Contains 9 WD repeats.
Cellular localizationCytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
- Information by UniProt
- Beta'-coat protein antibody
- Beta'-COP antibody
- Coatomer subunit beta' antibody
All lanes : Anti-COPB2 antibody (ab229639) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
All lanes : Goat anti-Rabbit at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 102, 99 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
Paraffin-embedded human spleen tissue stained for COPB2 with ab229639 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for COPB2 with ab229639 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229639 has not yet been referenced specifically in any publications.