Overview

  • Product name

    Anti-COPB2 antibody - C-terminal
    See all COPB2 primary antibodies
  • Description

    Rabbit polyclonal to COPB2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human COPB2 aa 856-906 (C terminal). The exact sequence is proprietary. NP_004757.1.
    Sequence:

    KPASP TPVIVASHTA NKEEKSLLEL EVDLDNLELE DIDTTDINLD EDILDD


    Database link: P35606

  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 whole cell lysates.
  • General notes

     This product was previously labelled as beta COP I

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7-8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab192924 was affinity purified using an epitope specific to COPB2 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/1000.

Target

  • Function

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
    This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.
  • Sequence similarities

    Belongs to the WD repeat COPB2 family.
    Contains 9 WD repeats.
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • Database links

  • Alternative names

    • Beta'-coat protein antibody
    • Beta'-COP antibody
    • Coatomer subunit beta' antibody
    • Copb2 antibody
    • COPB2_HUMAN antibody
    • p102 antibody
    see all

Images

  • Immunohistochemistry of human prostate carcinoma using ab192924 at 1/1000.

  • Immunohistochemistry of mouse renal cell carcinoma using ab192924 at 1/1000.

  • Detection of COPB2 by Western Blot of Immunprecipitate.
    Lane 1: ab192924 at 0.1 µg/ml labeling COPB2 in 293T whole cell lysate (prepared using NETN lysis buffer) immunoprecipitated using ab192924 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG.
    Detection: Chemiluminescence with exposure time of 30 seconds.

     

  • All lanes : Anti-COPB2 antibody - C-terminal (ab192924) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 102 kDa


    Exposure time: 30 seconds


    Lysates were prepared using NETN lysis buffer.

References

ab192924 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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