Key features and details
- Rabbit polyclonal to COPE
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COPE antibody
See all COPE primary antibodies
DescriptionRabbit polyclonal to COPE
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow, Chinese hamster, Orangutan
Recombinant full length protein corresponding to Human COPE aa 2-308.
APPAPGPASGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPERDVE RDVFLYRAYLAQRKFGVVLDEIKPSSAPELQAVRMFADYLAHESRRDSIV AELDREMSRSVDVTNTTFLLMAASIYLHDQNPDAALRALHQGDSLECTAM TVQILLKLDRLDLARKELKRMQDLDEDATLTQLATAWVSLATGGEKLQDA YYIFQEMADKCSPTLLLLNGQAACHMAQGRWEAAEGLLQEALDKDSGYPE TLVNLIVLSQHLGKPPEVTNRYLSQLKDAHRSHPFIKEYQAKENDFDRLV LQYAPSA
Database link: O14579
- WB: PC-3, HEK-293, HepG2, MCF-7, HeLa and A-375 whole cell lysates. IHC-P: Human prostate cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab235061 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/5000. Predicted molecular weight: 34 kDa.|
FunctionThe coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Sequence similaritiesBelongs to the COPE family.
modificationsPhosphorylated by PKA.
Polyubiquitinated by RCHY1 in the presence of androgen, leading to proteasomal degradation.
Cellular localizationCytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
- Information by UniProt
- 1110005D17Rik antibody
- Coatomer epsilon subunit antibody
- Coatomer protein complex subunit epsilon antibody
All lanes : Anti-COPE antibody (ab235061) at 1/500 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : A-375 (human malignant melanoma cell line) whole cell lysate
All lanes : Polyclonal Goat anti-rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Paraffin-embedded prostate cancer tissue stained for COPE using ab235061 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235061 has not yet been referenced specifically in any publications.