Overview

  • Product name

  • Description

    Rabbit polyclonal to COPE
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Chinese hamster, Orangutan
  • Immunogen

    Recombinant full length protein corresponding to Human COPE aa 2-308.
    Sequence:

    APPAPGPASGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPERDVE RDVFLYRAYLAQRKFGVVLDEIKPSSAPELQAVRMFADYLAHESRRDSIV AELDREMSRSVDVTNTTFLLMAASIYLHDQNPDAALRALHQGDSLECTAM TVQILLKLDRLDLARKELKRMQDLDEDATLTQLATAWVSLATGGEKLQDA YYIFQEMADKCSPTLLLLNGQAACHMAQGRWEAAEGLLQEALDKDSGYPE TLVNLIVLSQHLGKPPEVTNRYLSQLKDAHRSHPFIKEYQAKENDFDRLV LQYAPSA


    Database link: O14579

  • Positive control

    • WB: PC-3, HEK-293, HepG2, MCF-7, HeLa and A-375 whole cell lysates. IHC-P: Human prostate cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000. Predicted molecular weight: 34 kDa.

Target

  • Function

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
  • Sequence similarities

    Belongs to the COPE family.
  • Post-translational
    modifications

    Phosphorylated by PKA.
    Polyubiquitinated by RCHY1 in the presence of androgen, leading to proteasomal degradation.
  • Cellular localization

    Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.
  • Information by UniProt
  • Database links

  • Alternative names

    • 1110005D17Rik antibody
    • Coatomer epsilon subunit antibody
    • Coatomer protein complex subunit epsilon antibody
    • Coatomer subunit epsilon antibody
    • COPE antibody
    • COPE_HUMAN antibody
    • Cope1 antibody
    • Epsilon coat protein antibody
    • Epsilon COP antibody
    • Epsilon COP I antibody
    • Epsilon subunit of coatomer protein complex antibody
    • Epsilon-coat protein antibody
    • Epsilon-COP antibody
    • FLJ13241 antibody
    see all

Images

  • All lanes : Anti-COPE antibody (ab235061) at 1/500 dilution

    Lane 1 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
    Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
    Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 6 : A-375 (human malignant melanoma cell line) whole cell lysate

    Secondary
    All lanes : Polyclonal Goat anti-rabbit IgG at 1/50000 dilution

    Predicted band size: 34 kDa

  • Paraffin-embedded prostate cancer tissue stained for COPE using ab235061 at 1/100 dilution in immunohistochemical analysis.

References

ab235061 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab235061.
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