Recombinant Anti-COPE antibody [EPR12579] (ab180946)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-COPE antibody [EPR12579]
See all COPE primary antibodies -
Description
Rabbit monoclonal [EPR12579] to COPE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human COPE aa 50-150. The exact sequence is proprietary.
Database link: O14579 -
Positive control
- MCF-7, PC-3, HeLa and A375 cell lysate. Human liver tissue. MCF-7 and A375 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12579 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab180946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 34 kDa. | |
| IHC-P | 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50. | |
| Flow Cyt | 1/70. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. -
Sequence similarities
Belongs to the COPE family. -
Post-translational
modificationsPhosphorylated by PKA.
Polyubiquitinated by RCHY1 in the presence of androgen, leading to proteasomal degradation. -
Cellular localization
Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. - Information by UniProt
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Database links
- Entrez Gene: 11316 Human
- Omim: 606942 Human
- SwissProt: O14579 Human
- Unigene: 10326 Human
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Alternative names
- 1110005D17Rik antibody
- Coatomer epsilon subunit antibody
- Coatomer protein complex subunit epsilon antibody
see all
Images
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![Western blot - Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-212868-ab1809461.jpg)
Western blot - Anti-COPE antibody [EPR12579] (ab180946)All lanes : Anti-COPE antibody [EPR12579] (ab180946) at 1/5000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A375 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-212867-ab1809462.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPE antibody [EPR12579] (ab180946)Immunohistochemical analysis of paraffin embedded Human liver tissue sections labeling COPE using ab180946 at a 1/50 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-212866-ab1809463.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-COPE antibody [EPR12579] (ab180946)Immunofluorescent analysis of formaldehyde fixed MCF-7 cells labeling COPE using ab180946 at a 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) was used as the secondary at a 1/200 dilution. Counterstain DAPI.
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![Flow Cytometry - Anti-COPE antibody [EPR12579] (ab180946)](https://www.abcam.com/ps/products/180/ab180946/Images/ab180946-212865-ab1809464.jpg)
Flow Cytometry - Anti-COPE antibody [EPR12579] (ab180946)Flow Cytometry analysis of A375 cells labeling COPE using ab180946 at a 1/70 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Protocols
Datasheets and documents
References
ab180946 has not yet been referenced specifically in any publications.
