Overview

  • Product name

  • Description

    Rabbit polyclonal to COPS3/CSN3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Platypus
  • Immunogen

    Synthetic peptide corresponding to Human COPS3/CSN3.

  • Positive control

    • HEK 293T cells.
  • General notes

    Centrifuge tube to remove product from lid.

     This product was previously labelled as COPS3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab12321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/5000 - 1/25000. Predicted molecular weight: 51 kDa.

Target

  • Function

    Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • Tissue specificity

    Widely expressed. Expressed at high level in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the CSN3 family.
    Contains 1 PCI domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Constitutive photomorphogenic homolog subunit 3 antibody
    • COP 9 (constitutive photomorphogenic) subunit 3 antibody
    • COP 9 complex homolog subunit 3 antibody
    • COP 9 complex S3 antibody
    • COP 9 complex subunit 3 antibody
    • COP 9 constitutive photomorphogenic homolog subunit 3 antibody
    • COP 9 homolog antibody
    • COP 9 signalosome complex subunit 3 antibody
    • COP 9 signalosome subunit 3 antibody
    • COP 9 subunit 3 antibody
    • COP9 (constitutive photomorphogenic) subunit 3 antibody
    • COP9 complex homolog subunit 3 antibody
    • COP9 complex S3 antibody
    • COP9 complex subunit 3 antibody
    • COP9 constitutive photomorphogenic homolog subunit 3 antibody
    • COP9 homolog antibody
    • COP9 signalosome complex subunit 3 antibody
    • COP9 signalosome subunit 3 antibody
    • COP9 subunit 3 antibody
    • COPS 3 antibody
    • cops3 antibody
    • CSN 3 antibody
    • CSN3 antibody
    • CSN3_HUMAN antibody
    • JAB 1 containing signalosome subunit 3 antibody
    • JAB1 containing signalosome subunit 3 antibody
    • JAB1-containing signalosome subunit 3 antibody
    • SGN 3 antibody
    • SGN3 antibody
    • Signalosome subunit 3 antibody
    see all

Images

  • Immunohisotchemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling COPS3/CSN3 with ab12321 at 1/1000 (1µg/ml). Detection: DAB.

  • All lanes : Anti-COPS3/CSN3 antibody (ab12321) at 0.04 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Predicted band size: 51 kDa


    Exposure time: 10 seconds
  • Detection of Human CSN3 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T). Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.

    Detection of Human CSN3 by Western Blot.
    Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN3 expression construct (T).
    Antibody: Affinity purified rabbit anti-CSN3 antibody BL1059 used at the indicated concentrations.
    Detection: Chemiluminescence with a 10 second exposure.

References

This product has been referenced in:

  • Olma MH  et al. An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases. J Cell Sci 122:1035-44 (2009). WB ; Human . Read more (PubMed: 19295130) »
  • Oh W  et al. Jab1 induces the cytoplasmic localization and degradation of p53 in coordination with Hdm2. J Biol Chem 281:17457-65 (2006). Read more (PubMed: 16624822) »
See all 2 Publications for this product

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