Key features and details
- Rabbit polyclonal to COPS3/CSN3
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-COPS3/CSN3 antibody
See all COPS3/CSN3 primary antibodies
DescriptionRabbit polyclonal to COPS3/CSN3
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Cynomolgus monkey, Orangutan, Xenopus tropicalis
Recombinant fragment corresponding to Human COPS3/CSN3 aa 194-423.
GLKNFERALYFYEQAITTPAMAVSHIMLESYKKYILVSLILLGKVQQLPK YTSQIVGRFIKPLSNAYHELAQVYSTNNPSELRNLVNKHSETFTRDNNMG LVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIE DGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAM DQEITVNPQFVQKSMGSQEDDSGNKPSSYS
Database link: Q9UNS2-1
- WB: MCF7, HeLa, HEK-293T and HT-29 whole cell lysates; Mouse heart, brain and ovarian lysates. IHC-P: Human testis and heart tissues. IP: HeLa whole cell lysate.
This product was previously labelled as COPS3
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IP||1/200 - 1/2000.|
FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Tissue specificityWidely expressed. Expressed at high level in heart and skeletal muscle.
Sequence similaritiesBelongs to the CSN3 family.
Contains 1 PCI domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Constitutive photomorphogenic homolog subunit 3 antibody
- COP 9 (constitutive photomorphogenic) subunit 3 antibody
- COP 9 complex homolog subunit 3 antibody
All lanes : Anti-COPS3/CSN3 antibody (ab229807) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse brain lysate
Lane 7 : Mouse ovarian lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Paraffin-embedded human testis tissue stained for COPS3/CSN3 using ab229807 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human heart tissue stained for COPS3/CSN3 using ab229807 at 1/100 dilution in immunohistochemical analysis.
COPS3/CSN3 was immunoprecipitated from 500 µg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 4 µg ab229807.
Lane 1: Rabbit monoclonal IgG IP in HeLa whole cell lysate.
Lane 2: ab229807 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 20 µg.
For western blotting, an HRP-conjugated anti-rabbit IgG,specific to the non-reduced form of IgG was used as the secondary antibody at 1/50000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229807 has not yet been referenced specifically in any publications.