• Product name

    Anti-COPS3/CSN3 antibody [EPR3127]
    See all COPS3/CSN3 primary antibodies
  • Description

    Rabbit monoclonal [EPR3127] to COPS3/CSN3
  • Host species

  • Tested applications

    Suitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human COPS3/CSN3 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HT29 cell lysate HeLa cells SKBR-3 cell lysate 293T cell lysate
  • General notes

     This product was previously labelled as COPS3


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab79698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/40.
Flow Cyt 1/20 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
    • Tissue specificity

      Widely expressed. Expressed at high level in heart and skeletal muscle.
    • Sequence similarities

      Belongs to the CSN3 family.
      Contains 1 PCI domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Constitutive photomorphogenic homolog subunit 3 antibody
      • COP 9 (constitutive photomorphogenic) subunit 3 antibody
      • COP 9 complex homolog subunit 3 antibody
      • COP 9 complex S3 antibody
      • COP 9 complex subunit 3 antibody
      • COP 9 constitutive photomorphogenic homolog subunit 3 antibody
      • COP 9 homolog antibody
      • COP 9 signalosome complex subunit 3 antibody
      • COP 9 signalosome subunit 3 antibody
      • COP 9 subunit 3 antibody
      • COP9 (constitutive photomorphogenic) subunit 3 antibody
      • COP9 complex homolog subunit 3 antibody
      • COP9 complex S3 antibody
      • COP9 complex subunit 3 antibody
      • COP9 constitutive photomorphogenic homolog subunit 3 antibody
      • COP9 homolog antibody
      • COP9 signalosome complex subunit 3 antibody
      • COP9 signalosome subunit 3 antibody
      • COP9 subunit 3 antibody
      • COPS 3 antibody
      • cops3 antibody
      • CSN 3 antibody
      • CSN3 antibody
      • CSN3_HUMAN antibody
      • JAB 1 containing signalosome subunit 3 antibody
      • JAB1 containing signalosome subunit 3 antibody
      • JAB1-containing signalosome subunit 3 antibody
      • SGN 3 antibody
      • SGN3 antibody
      • Signalosome subunit 3 antibody
      see all


    • All lanes : Anti-COPS3/CSN3 antibody [EPR3127] (ab79698) at 1/20000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    • Immunofluorescence staining of HeLa cells with purified ab79698 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • Overlay histogram showing HeLa cells stained with ab79698 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79698, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.


    This product has been referenced in:

    • Zarich N  et al. The CSN3 subunit of the COP9 signalosome interacts with the HD region of Sos1 regulating stability of this GEF protein. Oncogenesis 8:2 (2019). Read more (PubMed: 30631038) »
    • Zhang F  et al. Novel oncogene COPS3 interacts with Beclin1 and Raf-1 to regulate metastasis of osteosarcoma through autophagy. J Exp Clin Cancer Res 37:135 (2018). Read more (PubMed: 29970115) »
    See all 4 Publications for this product

    Customer reviews and Q&As


    Thank you for contacting us.

    I can inform you that the immunogens for the requested antibodies are as follows:

    Anti-COPS3 antibody [EPR3127] (ab79698)

    Synthetic peptide taken from within the region 380 to the C-terminusof human COPS3.

    Anti-COPS3 antibody (ab82232)

    Synthetic peptide taken from within the region 107-136of human COPS3.

    If the customer needs any more detailed information please let me know what they need it for and I may be able to help. For example if they are trying to see if the antibodies will be likely to detect a particular isoform.

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

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