Recombinant Anti-CSN4 antibody [EPR7453] (ab139688)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7453] to CSN4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CSN4 antibody [EPR7453]
See all CSN4 primary antibodies -
Description
Rabbit monoclonal [EPR7453] to CSN4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CSN4 aa 1-100 (N terminal). The exact sequence is proprietary.
-
Positive control
- SH-SY5Y, HepG2, HeLa and PC3 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7453 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
Target
-
Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. -
Sequence similarities
Belongs to the CSN4 family.
Contains 1 PCI domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 51138 Human
- Entrez Gene: 26891 Mouse
- Entrez Gene: 360915 Rat
- SwissProt: Q9BT78 Human
- SwissProt: O88544 Mouse
- SwissProt: Q68FS2 Rat
- Unigene: 190384 Human
- Unigene: 957 Mouse
see all -
Alternative names
- CH4 antibody
- COP9 (constitutive photomorphogenic) homolog subunit 4 antibody
- COP9 complex homolog subunit 4 antibody
see all
Images
-
All lanes : Anti-CSN4 antibody [EPR7453] (ab139688) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab139688 has been referenced in 1 publication.
- Manjarrez JR et al. Hsp90-dependent assembly of the DBC2/RhoBTB2-Cullin3 E3-ligase complex. PLoS One 9:e90054 (2014). WB ; Human . PubMed: 24608665