Key features and details
- Rabbit polyclonal to COPS6
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-COPS6 antibody
See all COPS6 primary antibodies
DescriptionRabbit polyclonal to COPS6
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Cow, Human, Pig, Zebrafish, Orangutan
Synthetic peptide corresponding to Human COPS6 aa 150-250.
(Peptide available as
- This antibody gave a positive signal in NIH3T3 whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab77299 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Has some glucocorticoid receptor-responsive activity.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the peptidase M67A family. CSN6 subfamily.
Contains 1 MPN (JAB/Mov34) domain.
Cellular localizationNucleus. Cytoplasm. The interaction with HIV-1 Vpr protein possibly leads its translocation to a perinuclear region.
- Information by UniProt
- COP9 (constitutive photomorphogenic), subunit 6; COP9 complex S6 antibody
- COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis) antibody
- COP9 signalosome complex subunit 6 antibody
Anti-COPS6 antibody (ab77299) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 20 minutes
ICC/IF image of ab77299 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77299, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293 and HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
ab77299 has been referenced in 1 publication.
- Deng Q et al. CSN6 and Rab34 Are Involved in Androgen Receptor Trafficking in Mouse Testicular Sertoli Cells. Cell Physiol Biochem 47:2360-2368 (2018). PubMed: 29991022