Overview

  • Product name

  • Description

    Rabbit polyclonal to COPT2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Recombinant fragment
    Predicted to work with: Mouse, Human
  • Immunogen

    A synthetic peptide corresponding to C-terminal residues of human COPT2

Properties

Applications

Our Abpromise guarantee covers the use of ab58777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

Target

  • Function

    Involved in low-affinity copper uptake.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI604396 antibody
    • Copper transporter 2 antibody
    • COPT2 antibody
    • COPT2_HUMAN antibody
    • CTR2 antibody
    • hCTR2 antibody
    • MGC117640 antibody
    • Probable low affinity copper uptake protein 2 antibody
    • RP23 64F17.2 antibody
    • SLC31A2 antibody
    • Solute carrier family 31 member 2 antibody
    • Solute carrier family 31, member 2 antibody
    see all

Images

  • ICC/IF image of ab58777 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58777, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Cai J  et al. Simultaneous overactivation of Wnt/ß-catenin and TGFß signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC. Nat Commun 8:15870 (2017). Read more (PubMed: 28627514) »
See 1 Publication for this product

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