Key features and details
- Rabbit polyclonal to COQ4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab126295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
FunctionComponent of the coenzyme Q biosynthetic pathway. May play a role in organizing a multi-subunit COQ enzyme complex required for coenzyme Q biosynthesis. Required for steady-state levels of other COQ polypeptides.
Tissue specificityExpressed ubiquitously, but at high levels in liver, lung and pancreas.
PathwayCofactor biosynthesis; ubiquinone biosynthesis.
Sequence similaritiesBelongs to the COQ4 family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- CGI 92 antibody
- Coenzyme Q biosynthesis protein 4 homolog antibody
- coq4 antibody
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
ab126295, at a 1/1000 dilution, staining COQ4 in paraffin embedded Human pancreas tissue by Immunohistochemistry.
ab126295 has been referenced in 1 publication.
- Rooney MF et al. Skeletal muscle mitochondrial bioenergetics and associations with myostatin genotypes in the Thoroughbred horse. PLoS One 12:e0186247 (2017). PubMed: 29190290