Recombinant Anti-CoREST antibody [EPR13825] (ab183711)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13825] to CoREST
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CoREST antibody [EPR13825]
See all CoREST primary antibodies -
Description
Rabbit monoclonal [EPR13825] to CoREST -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Molt-4 and Jurkat cell lysates; Human cervix carcinoma and Rat colon tissues; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13825 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
Target
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Function
Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the CoREST family.
Contains 1 ELM2 domain.
Contains 2 SANT domains. -
Domain
The SANT domains may bridge the nucleosomal substrates and the demethylase KDM1A. -
Post-translational
modificationsPhosphorylated by HSV-1 protein kinases in case of infection. -
Cellular localization
Nucleus. Upon infection by HSV-1, it is partially translocated into the cytoplasm in an HSV-1-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 23186 Human
- Entrez Gene: 217864 Mouse
- Entrez Gene: 314458 Rat
- Omim: 607675 Human
- SwissProt: Q9UKL0 Human
- SwissProt: Q8CFE3 Mouse
- SwissProt: P49743 Rat
- Unigene: 510521 Human
see all -
Alternative names
- COREST antibody
- KIAA0071 antibody
- Nr2b2 antibody
see all
Images
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All lanes : Anti-CoREST antibody [EPR13825] (ab183711) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : MOLT-4 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling CoREST with ab183711 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling CoREST with ab183711 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunofluorescent analysis of acetone-fixed HeLa cells labeling CoREST with ab183711 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab183711 has been referenced in 4 publications.
- Rivera C et al. Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin. Nat Commun 13:1550 (2022). PubMed: 35322029
- Tu WJ et al. Targeting novel LSD1-dependent ACE2 demethylation domains inhibits SARS-CoV-2 replication. Cell Discov 7:37 (2021). PubMed: 34031383
- Mehta SL et al. Long Noncoding RNA FosDT Promotes Ischemic Brain Injury by Interacting with REST-Associated Chromatin-Modifying Proteins. J Neurosci 35:16443-9 (2015). WB ; Rat . PubMed: 26674869
- Chan FC et al. An RCOR1 loss-associated gene expression signature identifies a prognostically significant DLBCL subgroup. Blood 125:959-66 (2015). WB ; Human . PubMed: 25395426