Overview

  • Product name

    Anti-CoREST antibody [EPR13825]
    See all CoREST primary antibodies
  • Description

    Rabbit monoclonal [EPR13825] to CoREST
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CoREST aa 1-100. The exact sequence is proprietary.
    Database link: Q9UKL0

  • Positive control

    • HeLa, Molt-4 and Jurkat cell lysates; Human cervix carcinoma and Rat colon tissues; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13825
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/50.
ICC/IF 1/100.

Target

  • Function

    Essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the CoREST family.
    Contains 1 ELM2 domain.
    Contains 2 SANT domains.
  • Domain

    The SANT domains may bridge the nucleosomal substrates and the demethylase KDM1A.
  • Post-translational
    modifications

    Phosphorylated by HSV-1 protein kinases in case of infection.
  • Cellular localization

    Nucleus. Upon infection by HSV-1, it is partially translocated into the cytoplasm in an HSV-1-dependent manner.
  • Information by UniProt
  • Database links

  • Alternative names

    • COREST antibody
    • KIAA0071 antibody
    • Nr2b2 antibody
    • Nuclear receptor coregulator 1 antibody
    • Nuclear receptor subfamily 2 group B member 2 antibody
    • Protein CoREST antibody
    • RCOR antibody
    • Rcor1 antibody
    • RCOR1_HUMAN antibody
    • REST corepressor 1 antibody
    • Retinoic acid receptor RXR-beta antibody
    • Retinoid X receptor beta antibody
    • Rxrb antibody
    see all

Images

  • All lanes : Anti-CoREST antibody [EPR13825] (ab183711) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Molt-4 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 66 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling CoREST with ab183711 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling CoREST with ab183711 at 1/50 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin

  • Immunofluorescent analysis of acetone-fixed HeLa cells labeling CoREST with ab183711 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution (green). Counter stained with Dapi (blue).

References

This product has been referenced in:

  • Mehta SL  et al. Long Noncoding RNA FosDT Promotes Ischemic Brain Injury by Interacting with REST-Associated Chromatin-Modifying Proteins. J Neurosci 35:16443-9 (2015). WB ; Rat . Read more (PubMed: 26674869) »
  • Chan FC  et al. An RCOR1 loss-associated gene expression signature identifies a prognostically significant DLBCL subgroup. Blood 125:959-66 (2015). WB ; Human . Read more (PubMed: 25395426) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - nuclear (Hippocampus, liver and MCF7 whole cell hpmogenates)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
80 µg
Specification
Hippocampus, liver and MCF7 whole cell hpmogenates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Octavio Mercado-Gomez

Verified customer

Submitted May 12 2016

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